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首页> 外文期刊>The journal of physical chemistry, A. Molecules, spectroscopy, kinetics, environment, & general theory >Photobleaching of Rhodamine 6G in Poly(vinyl alcohol) at the Ensemble and Single-Molecule Levels
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Photobleaching of Rhodamine 6G in Poly(vinyl alcohol) at the Ensemble and Single-Molecule Levels

机译：整体和单分子水平的若丹明6G在聚乙烯醇中的光漂白

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摘要

Photobleaching is a severely limiting factor in the optical study of single biomolecules. We investigate the photobleaching of rhodamine 6G (R6G) ensembles in poly(vinyl alcohol) (PVA) as a function of illumination time, excitation intensity, the presence of oxygen, and temperature. We observe nonexponential kinetics related to primary photobleaching through two dark states-the triplet state and a radical anion-and to secondary photobleaching after the optical excitation of those dark states. Reactions of the metastable states with oxygen can lead either to photoproducts or to a recovery of the ground state. Oxygen can therefore enhance or reduce photobleaching, depending on the experimental conditions. At low temperature, photobleaching is reduced although not completely suppressed. Despite the presence of the long-lived radical anion, we are able to observe single R6G molecules in PVA. At room temperature, only relatively bleaching-resistant molecules are resolved as individuals. At low temperature, the observation times become considerably longer. Our study shows that metastable states other than the triplet drastically affect photobleaching.
机译：光漂白是单个生物分子光学研究中的一个严格限制因素。我们研究了照明时间，激发强度，氧气的存在和温度对聚（乙烯醇）（PVA）中罗丹明6G（R6G）团簇的光致漂白的影响。我们观察到与通过两个暗态（三重态和一个自由基阴离子）的初级光漂白有关的非指数动力学，以及在这些暗态的光激发后与次级光漂白有关的非指数动力学。亚稳态与氧的反应可能导致光产物或基态的恢复。因此，取决于实验条件，氧气可以增强或减少光致漂白。在低温下，虽然没有完全抑制光漂白，但是减少了漂白。尽管存在长寿命的自由基阴离子，我们仍然能够在PVA中观察到单个R6G分子。在室温下，只有相对抗漂白的分子才能分解为单个分子。在低温下，观察时间变得相当长。我们的研究表明，除了三重态以外，亚稳态还极大地影响了光致漂白。

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