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首页> 外文期刊>The journal of physical chemistry, A. Molecules, spectroscopy, kinetics, environment, & general theory >Correlations of Structure and Rates of Energy Transfer for Through-Bond Energy-Transfer Cassettes
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Correlations of Structure and Rates of Energy Transfer for Through-Bond Energy-Transfer Cassettes

机译：跨境能量转移盒的能量转移结构和速率的相关性

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摘要

Fluorescent DNA-labeling cassettes are designed to have a common absorbing chromophore matched to a single exciting laser wavelength,but up to four different emitters. Experiments reported here have examined the energy-transfer rates and fluorescence polarization characteristics for two different types of cassette,involving three distinct relative orientations of the donor and acceptor transition moments and the axis of the rigid linker. Energy-transfer times range from <200 fs to approx 20 ps,the fastest transfer times occurring when the transition moments of the donor and acceptor species are aligned parallel to the linker axis. Experimental evidence is presented that supports a through-bond energy-transfer mechanism,in contrast with a commercial DNA-labeling agent,which exhibits much slower transfer times controlled by FRET. These rigid cassettes also exhibit polarized fluorescence from the acceptor species,so that this particular type of DNA-labeling probe has some of the advantages of single-molecule probes such as rhodamine and coumarin dyes.
机译：荧光DNA标记盒设计为具有与单个激发激光波长匹配的通用吸收发色团，但最多可以有四个不同的发射体。此处报道的实验检查了两种不同类型盒的能量传递速率和荧光偏振特性，涉及供体和受体跃迁矩和刚性接头的轴的三个不同的相对方向。能量传递时间范围从<200 fs到大约20 ps，这是最快的传递时间，发生在施主和受主物种的跃迁矩平行于连接体轴对齐时。实验证明，与商业化的DNA标记剂相比，它支持通过键的能量转移机制，而后者在FRET的控制下表现出慢得多的转移时间。这些刚性盒也显示出来自受体物种的偏振荧光，因此这种特定类型的DNA标记探针具有罗丹明和香豆素染料等单分子探针的某些优势。

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