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首页> 外文期刊>The journal of physical chemistry, A. Molecules, spectroscopy, kinetics, environment, & general theory >Tracking the Photosensitizing Antibacterial Activity of Mono(acridyl)bis(arginyl)porphyrin (MABAP) by Time-Resolved Spectroscopy
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Tracking the Photosensitizing Antibacterial Activity of Mono(acridyl)bis(arginyl)porphyrin (MABAP) by Time-Resolved Spectroscopy

机译：时间分辨光谱跟踪单（ac啶基）双（精氨酰基）卟啉（MABAP）的光敏抗菌活性

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摘要

Photodynamic inactivation (PDI) is currently receiving interest for its potential as an antimicrobial treatment. Although photosensitizing agents and light have been used for medical purposes for a very long time, only a little information is available about the mechanism of PDI for bacteria. Pseudomonas aeruginosa is a Gram negative bacteria involved in chronic infections in cystic fibrosis patients and also one of the commonest agents of hospital acquired infections. In the present study the sensitivity of Pseudomonas aeruginosa to the phototoxic effects of the mono(acridyl)bis(arginyl)porphyrin (MABAP) has been investigated as well as the photophysical and photochemical properties of this cationic porphyrin complexed to [poly(dG-dC)]2 to investigate the mechanisms that lead to bacteria inactivation. Both picosecond time-resolved fluorescence and femtosecond to nanosecond transient absorption measurements give evidence that while MABAP can react through its triplet state and/or an ultrafast electron transfer with guanine, its intercalation between GC base pairs is not the main target of MABAP photoactivity. The analysis of both fluorescence emission and excitation spectra reveals the occurrence of an energy transfer through the DNA double helix between the acridine and porphyrin chromophores of MABAP, as previously observed for the stacked free molecule in solution. This efficient process may lead to the excitation of twice more porphyrin chromophores in MABAP by comparison to other cationic porphyrins.
机译：目前，光动力灭活（PDI）作为抗微生物治疗的潜力受到了人们的关注。尽管光敏剂和光在医学上已经使用了很长时间，但是关于细菌PDI机理的信息很少。铜绿假单胞菌是革兰氏阴性细菌，与囊性纤维化患者的慢性感染有关，也是医院获得性感染的最常见媒介之一。在本研究中，已研究了铜绿假单胞菌对单（）啶基）双（精氨酰基）卟啉（MABAP）的光毒性作用的敏感性，以及与[聚（dG-dC）络合的阳离子卟啉的光物理和光化学性质。 ）] 2研究导致细菌失活的机制。皮秒时间分辨的荧光和飞秒至纳秒的瞬态吸收测量都提供了证据，尽管MABAP可以通过其三重态和/或与鸟嘌呤的超快电子转移发生反应，但它在GC碱基对之间的插入并不是MABAP光活性的主要目标。荧光发射光谱和激发光谱的分析表明，通过MABAP的a啶和卟啉发色团之间的DNA双螺旋发生了能量转移，正如先前在溶液中堆积的自由分子所观察到的。与其他阳离子卟啉相比，此有效过程可能导致MABAP中的卟啉发色团多两倍。

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