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首页> 外文期刊>The journal of physical chemistry, A. Molecules, spectroscopy, kinetics, environment, & general theory >Analysis of the Spectroscopic Aspects of Cationic Dye Basic Orange 21
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Analysis of the Spectroscopic Aspects of Cationic Dye Basic Orange 21

机译：阳离子染料碱性橙21的光谱分析

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摘要

Spectroscopic properties of cationic dye basic orange 21 (BO21) in solutions, in solids, and within leukocytes were examined. Results obtained with solutions indicate that influence of variables such as pH, viscosity, salt composition, and various proteins on the absorption spectrum of BO21 is negligible. However, in the presence of heparin, a blue shift (484-465 nm) is observed, which is attributed to the aggregation of BO21 on the polyanion. Applying density functional theory demonstrates that in aqueous solutions (a) the formation of BO21 oligomers is thermodynamically favorable, they are oriented in an antiparallel dipolar arrangement, and their binding energies are lower than those of parallel dipolar arrangements, (b) association between BO21 aggregates and heparin is highly favorable, and (c) the blue shift is due to the mixing of pi -> pi* transitions caused by BO21 molecule stacking. However, when embedded in basophils, the absorption spectra of intracellular BO21 is extremely red-shifted, with two peaks (at 505 and 550 nm) found to be attributed to BO21 and the BO21 heparin complex, respectively, which are intracellularly hosted in nonaqueous environments. Initial evidence of the ability to differentiate between leukocyte types by BO21 is presented.
机译：检查了阳离子染料碱性橙21（BO21）在溶液中，固体中和白细胞中的光谱性质。用溶液获得的结果表明，pH，粘度，盐组成和各种蛋白质等变量对BO21吸收光谱的影响可以忽略不计。然而，在肝素的存在下，观察到蓝移（484-465nm），这归因于BO21在聚阴离子上的聚集。应用密度泛函理论证明，在水溶液中（a）BO21低聚物的形成在热力学上是有利的，它们以反平行偶极排列取向，并且其结合能比平行偶极排列的结合能低，（b）BO21聚集体之间的缔合（c）蓝移是由于BO21分子堆积引起的pi-> pi *跃迁的混合所致。但是，当包埋在嗜碱性粒细胞中时，细胞内BO21的吸收光谱会发生极大的红移，发现两个峰（分别在505和550 nm处）分别归因于BO21和BO21肝素复合物，它们分别在非水环境中存在于细胞内。 。提出了通过BO21区分白细胞类型的能力的初步证据。

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