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A Comparison of Single-Molecule Emission in Aluminum and Gold Zero-Mode Waveguides

机译:铝和金零模波导中单分子发射的比较

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The effect of gold and aluminum zero-mode waveguides (ZMWs) on the brightness of immobilized Single emitters was characterized by probing fluorophores that absorb in the green. and red regions of the visible spectrum. Aluminum ZMWs enhance the emission of Atto565 fluorophores upon green excitation, but they do not enhance the emission of Atto647N fluorophores upon red excitation. Gold ZMWs increase emission of both fluorophores with Atto647N showing enhancement that is threefold higher than that observed for Atto565. This work indicates that 200 nm gold ZMWs are better suited for Single Molecule fluorescence studies the red region of:the visible spectrum, while aluminum appears more-suited for the green region of the visible spectrum.
机译:金和铝零模波导(ZMW)对固定的单发射器亮度的影响是通过探测吸收绿色的荧光团来表征的。和可见光谱的红色区域。铝ZMW在绿色激发时增强Atto565荧光团的发射,但在红色激发时它们不增强Atto647N荧光团的发射。金的ZMW可以增加两个荧光团的发射,而Atto647N的发射增强是Atto565观察到的增强的三倍。这项工作表明200 nm金ZMWs更适合单分子荧光研究可见光谱的红色区域,而铝似乎更适合可见光谱的绿色区域。

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