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首页> 外文期刊>The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical >Detection of adsorption of Ru(II) and Os(II) polypyridyl complexes on gold and silver nanoparticles by single-photon counting emission measurements
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Detection of adsorption of Ru(II) and Os(II) polypyridyl complexes on gold and silver nanoparticles by single-photon counting emission measurements

机译：通过单光子计数发射测量检测Ru（II）和Os（II）聚吡啶基配合物在金和银纳米颗粒上的吸附

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摘要

The present study describes a new application of ruthenium(II) tris(bipyridine) (Ru(bpy)(3)(2+)) and osmium(II) tris(bipyridine) (Os(bpy)(3)(2+)) as phosphorescent labels for the quantification of surface binding of molecules to gold and silver nanoparticles. The fraction of Rtt(bpy)(3)(2+) and Os(bpy)(3)(2+) that is in solution can be distinguished from the surface-bound fraction by the relative lifetimes and integrated emission yields as determined by time-correlated single-photon counting (TCSPC) spectroscopy. Complementary steady-state measurements were carried out to confirm surface attachment of the phosphorescent label molecules. Although the emission of solutions of Ru(bpy)(3)(2+) and Os(bpy)(3)(2+) is quenched proportional to the concentration of 10 nm Au or 20 nm Ag nanoparticles, the quenching is static and not diffusional quenching observed in Stern-Volmer plots. The results demonstrate that time-resolved spectroscopy provides a rapid method for the measurement of surface binding of labeled molecules on metallic nanoparticles. While steady-state measurements require the preparation of a series of samples with varying quencher concentrations and a reference, the method described herein requires a single sample plus reference. The mechanism for phosphorescence quenching on Au and Ag nanoparticles is discussed in terms of energy and electron transfer theories.
机译：本研究描述了钌（II）三（联吡啶）（Ru（bpy）（3）（2+））和（II）三联（联吡啶）（Os（bpy）（3）（2+） ）作为磷光标记，用于定量分子与金和银纳米颗粒的表面结合。溶液中的Rtt（bpy）（3）（2+）和Os（bpy）（3）（2+）的分数可以通过相对寿命和积分发射率与表面结合分数区别开时间相关的单光子计数（TCSPC）光谱。进行补充稳态测量以确认磷光标记分子的表面附着。尽管Ru（bpy）（3）（2+）和Os（bpy）（3）（2+）溶液的发射与10 nm Au或20 nm Ag纳米颗粒的浓度成比例地淬灭，但淬灭是静态的，并且在Stern-Volmer图中未观察到扩散猝灭。结果表明，时间分辨光谱法提供了一种快速的方法来测量金属纳米颗粒上标记分子的表面结合。虽然稳态测量需要准备一系列具有变化的淬灭剂浓度的样品和参考物，但本文所述的方法需要单个样品加参考物。根据能量和电子转移理论讨论了Au和Ag纳米粒子上磷光猝灭的机理。

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