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首页> 外文期刊>The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical >Detection of Bilayer Packing Stress and Its Release in Lamellar-Cubic Phase Transition by Time-Resolved Fluorescence Anisotropy
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Detection of Bilayer Packing Stress and Its Release in Lamellar-Cubic Phase Transition by Time-Resolved Fluorescence Anisotropy

机译：时间分辨荧光各向异性检测双层堆积应力及其在层状-立方相变中的释放

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摘要

An introduction of nonlamellar-forming lipids into planar bilayers generates packing stress,which is important for the biological functions of plasma membranes and is a driving force for the lamellar-nonlamellar phase transition.We have investigated the phase behavior of a binary system consisting of egg yolk phosphatidyl-choline and monoolein (MO) and the changes in the local orientation order of lipids in a lamellar-bicontinuous cubic phase transition.Small-angle X-ray scattering has revealed that the lamellar-bicontinuous cubic phase transition occurs at an MO molar fraction (X_(MO)) between 0.6 and 0.7.These phases were dispersed to form liposomes and cubosomes to monitor the anisotropy of the incorporated fluorescence probe,in which Pluronic F127,used as a dispersion stabilizer of the cubic phase,has been proven not to alter the cubic structure and the location of the probes.Time-resolved fluorescence anisotropy measurements on these dispersions have revealed that the order parameter of the probe in the lamellar phase increases with increasing X_(MO),and that it decreases during the transition to the cubic phase.This observation suggests that packing stress generated by the addition of the nonlamellar-forming lipid is released by the phase transition.
机译：将非层状脂质引入平面双层中会产生堆积应力，这对于质膜的生物学功能很重要，并且是层状非层状相变的驱动力。我们研究了由卵​​组成的二元体系的相态卵黄磷脂酰胆碱和单油精（MO）以及层状-双连续立方相变中脂质的局部取向顺序变化。小角度X射线散射表明，层状-双连续立方相转变发生在MO摩尔分数（X_（MO））在0.6到0.7之间。这些相分散形成脂质体和立方体，以监测掺入的荧光探针的各向异性，其中Pluronic F127用作立方相的分散稳定剂，已被证明没有这些分散体的时间分辨荧光各向异性测量结果表明，有序参数可用于改变探针的立方结构和位置。层状相中探针的ter随X_（MO）的增加而增加，并且在过渡到立方相时降低。该观察结果表明，相变释放了由添加非层状脂质产生的堆积应力。

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