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Electrostatic stitching in gel-phase supported phospholipid bilayers

机译:凝胶相支持的磷脂双层中的静电缝合

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摘要

We show that mixing zwitterionic lipids with up to 20% mole % cationic lipids produces gel-phase supported lipid bilayers that are morphologically free of defects detectable using noncontact mode atomic force microscopy (AFM). This contrasts with the observation of massive defects when anionic lipid was added, and also when no charged lipid was added. Infrared measurements of headgroup orientation in the presence of cationic lipid show that the mean headgroup orientation changes only minimally when temperature is lowered from the fluid phase to the gel phase. This is consistent with a tentative explanation, based on simple electrostatic arguments, in which cationic lipids "stitch" the bilayers together. On the functional side, this study demonstrates a simple method by which to minimize defects in gel-supported phospholipid bilayers.
机译:我们显示,将两性离子脂质与高达20%摩尔%的阳离子脂质混合会产生凝胶相支持的脂质双层,该双层在形态上没有可使用非接触模式原子力显微镜(AFM)检测到的缺陷。这与在添加阴离子脂质以及未添加带电荷脂质时观察到大量缺陷的情况相反。在存在阳离子脂质的情况下对头基取向的红外测量表明,当温度从液相降低到凝胶相时,平均头基取向的变化很小。这与基于简单的静电学说的初步解释一致,其中阳离子脂质将双层“缝合”在一起。在功能方面,这项研究证明了一种简单的方法,可通过该方法最大程度地减少凝胶支持的磷脂双层中的缺陷。

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