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首页> 外文期刊>The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical >The Role of Different Structural Motifs in the Ultrafast Dynamics of Second Generation Protein Stains
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The Role of Different Structural Motifs in the Ultrafast Dynamics of Second Generation Protein Stains

机译：不同结构基元在第二代蛋白质污渍超快动力学中的作用

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Engineering the properties of fluorescent probes through modifications of the fluorophore structure has become a subject of interest in recent times. By doing this, the photophysical and photochemical properties of the modified fluorophore can be understood and this can guide the design and synthesis of better fluorophores for use in biotechnology. In this work, the electronic spectra and fluorescence decay kinetics of four analogues of the fluorescent natural product epicocconone were investigated. Epicocconone is unique in that the native state is weakly green fluorescent, whereas the enamine formed reversibly with proteins is highly emissive in the red. It was found that the ultrafast dynamics of the analogues depends profoundly on the H-bonding effect of solvents and solvent viscosity though solvent polarity also plays a role. Comparing the steady state and time-resolved data, the weak fluorescence of epicocconone in its native state is most likely due to the photoisomerization of the hydrocarbon side chain, while the keto enol moiety also has a role to play in determining the fluorescence quantum yield. This understanding is expected to aid the design of better protein stains from the same family.
机译：通过改变荧光团结构来工程化荧光探针的性质已成为近来关注的主题。通过这样做，可以理解修饰的荧光团的光物理和光化学性质，这可以指导用于生物技术的更好的荧光团的设计和合成。在这项工作中，研究了荧光天然产物表环可可酮的四个类似物的电子光谱和荧光衰减动力学。 Epicocconone的独特之处在于其天然状态呈弱绿色荧光，而与蛋白质可逆形成的烯胺则呈红色高发射。已发现，尽管溶剂极性也起作用，但类似物的超快动力学很大程度上取决于溶剂的氢键作用和溶剂粘度。比较稳态数据和时间分辨数据，表皮康酮在其原始状态下的弱荧光最有可能是由于烃侧链的光异构化引起的，而酮烯醇部分也起着决定荧光量子产率的作用。期望这种理解有助于设计来自同一家族的更好的蛋白质染色剂。

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