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首页> 外文期刊>The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical >Determination of Sorption Isotherm and Rheological Properties of Lysozyme Using a High-Resolution Humidity Scanning QCM-D Technique
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Determination of Sorption Isotherm and Rheological Properties of Lysozyme Using a High-Resolution Humidity Scanning QCM-D Technique

机译：高分辨率湿度扫描QCM-D技术测定溶菌酶的吸附等温线和流变性质

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摘要

The high-resolution humidity scanning QCM- D technique enables investigation of hydration of soft matter films using a quartz crystal microbalance with dissipation monitoring (QCM-D) equipped with a humidity module. Based on a continuous increase of relative humidity, properties of soft matter films can be investigated depending on the water content of the surrounding atmosphere. Determination of complete water sorption isotherms is possible via analysis of the overtone dependence of the resonance frequencies. Rheological properties are monitored via measurement of the dissipation. The glass transition can be identified from the change of viscoelastic properties of the film reflected in changes of the dissipation. A high-resolution water sorption isotherm of lysozyme was measured and compared with results from water sorption calorimetry. Analysis of the rheological behavior during hydration of lysozyme films revealed the presence of two separate sharp transitions at the water activities 0.67 and 0.91, which are connected to the glass transition. In previous works, only the existence of a broad glass transition has been reported so far. Combining the QCM-D data with Raman scattering data presented earlier, a new mechanism of isothermal glass transition in lysozyme is proposed.
机译：高分辨率湿度扫描QCM-D技术可使用配备了湿度模块的耗散监控（QCM-D）石英晶体微天平来研究软质薄膜的水合作用。基于相对湿度的持续增加，可以根据周围大气中的水分来研究软质薄膜的性能。通过分析共振频率的泛音依赖性，可以确定完全的水吸附等温线。通过测量耗散来监测流变性能。可以从耗散变化中反映出的膜的粘弹性特性的变化来识别玻璃化转变。测量了溶菌酶的高分辨率水吸附等温线，并将其与水吸附量热法的结果进行了比较。分析溶菌酶薄膜水化过程中的流变行为表明，在水活度0.67和0.91处存在两个独立的急剧转变，这两个转变与玻璃化转变有关。迄今为止，在以前的著作中，仅报道了广泛的玻璃化转变。结合先前提出的QCM-D数据和拉曼散射数据，提出了溶菌酶等温玻璃化转变的新机理。

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