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首页> 外文期刊>The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical >Biocolloids Based on Amphiphilic Block Copolymers as a Medium for Enzyme Encapsulation
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Biocolloids Based on Amphiphilic Block Copolymers as a Medium for Enzyme Encapsulation

机译：基于两亲嵌段共聚物作为酶包封介质的生物胶体

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摘要

The ability of two biocompatible amphiphilic block copolymers consisting of hydrophilic poly(ethylene oxide) and hydrophobic poly(ε-caprolactone) with different hydrophilic/hydrophobic block ratio to act as stabilizers of* water-in-oil (w/o) microemulsions and enzyme encapsulation therein has been tested. Phase diagrams of the two block copolymers in mixtures of chloroform/isopropanol/water were constructed, revealing that the systems can incorporate important amounts of aqueous phase. The w/o microemulsions were then used to encapsulate R. miehei lipase. Empty as well as lipase-loaded systems were characterized by DLS as well as EPR spectroscopy. It was found that the incorporated lipase was preferably localized in the interior of the droplets. The apparent hydrodynamic radii of the droplets were found to vaiy from 86 to 3000 nm and from 66 to 2140 nm for empty PEO-PCL 30 and PEO-PCL 53 stabilized systems, respectively. In the presence of the lipase, the hydrodynamic radii were considerably decreased. The catalytic activity of the encapsulated lipase was successfully tested via a model esterification reaction. The effect of temperature on the catalytic behavior of the encapsulated R. miehei lipase was investigated, revealing that the initial rate of the esterification reaction depended on the type of the block copolymer used.
机译：具有不同亲水/疏水嵌段比的亲水性聚环氧乙烷和疏水性聚ε-己内酯组成的两种生物相容性两亲嵌段共聚物作为油包水微乳液和酶的稳定剂的能力其中的封装已经过测试。绘制了两种嵌段共聚物在氯仿/异丙醇/水混合物中的相图，表明该体系可以引入大量的水相。然后将w / o微乳剂用于封装米黑麦芽孢杆菌脂肪酶。用DLS和EPR光谱表征空的以及负载脂肪酶的系统。发现掺入的脂肪酶优选位于液滴的内部。对于空的PEO-PCL 30和PEO-PCL 53稳定系统，发现液滴的表观流体力学半径分别为86至3000 nm和66至2140 nm。在脂肪酶的存在下，流体动力学半径显着降低。通过模型酯化反应成功地测试了包封的脂肪酶的催化活性。研究了温度对封装的米黑霉脂肪酶催化性能的影响，发现酯化反应的初始速率取决于所用嵌段共聚物的类型。

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