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首页> 外文期刊>The journal of physical chemistry, C. Nanomaterials and interfaces >Photosynthetic Bacterial Light-Harvesting Antenna Complexes Adsorbed on Silica Nanoparticles Revealed by Silica Shell-lsobted Au Nanoparticle-Enhanced Raman Spectroscopy
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Photosynthetic Bacterial Light-Harvesting Antenna Complexes Adsorbed on Silica Nanoparticles Revealed by Silica Shell-lsobted Au Nanoparticle-Enhanced Raman Spectroscopy

机译：二氧化硅壳固溶金纳米增强拉曼光谱揭示的二氧化硅纳米颗粒上吸附的光合细菌光捕获天线复合物

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Assembling of photosynthetic light-harvesting proteins with inorganic nanoparticles can be a promising strategy for developing artificial solar cells. However, characterization of protein adsorption and its spatial orientation with respect to the interface is still a challenging task. Here we . report surface-enhanced Raman spectra of light-harvesting antenna complexes (LH2) from two different photosynthetic bacteria adsorbed onto Au core silica shell (Au(S)SiO2) nanoparticles to illustrate LH2 adsorption and orientation at the interface. The surface plasmon enhanced Raman spectra of typical vibrational modes of carotenoids are used as the intrinsic reporter of LH2 adsorbed on Au@SiO2. Carotenoid molecules are asymmetrically located inside the disk-like LH2 closest to cytoplasmic side, leading to an orientation-dependent surface-enhanced Raman gain when the LH2 binds to the interface with one of its two end surfaces. We find that the Raman gain factors for LH2 from Rba. sphaeroides are larger than those from Rps. acidophila, suggesting that these two different LH2s adsorbed on the negatively charged silica shell with opposite orientation of their end surfaces carrying opposite charges. This is further supported by the observed difference in the BS50 excited-state lifetime change for these two different LH2s when adsorbed onto silica nanoparticles, where the lifetime of B850 acts as an intrinsic reporter for nanoparticle-induced protein deformation which is also orientation-dependent, owing to the asymmetrical location of B850 ring inside the LH2.
机译：将光合光捕获蛋白与无机纳米粒子组装在一起可能是开发人造太阳能电池的有前途的策略。然而，表征蛋白质吸附及其相对于界面的空间取向仍然是一项艰巨的任务。在这里，我们 。报告了来自两个不同的光合细菌的光捕获天线复合物（LH2）的表面增强拉曼光谱，这些光合细菌吸附在Au核心二氧化硅壳（Au（S）SiO2）纳米颗粒上，以说明LH2的吸附和界面取向。类胡萝卜素典型振动模式的表面等离子体激元增强拉曼光谱被用作吸附在Au @ SiO2上的LH2的本征报告物。类胡萝卜素分子不对称地位于最接近细胞质侧的盘状LH2内部，当LH2与其两个端面之一结合到界面时，导致取向依赖性的表面增强拉曼增益。我们发现来自Rba的LH2的拉曼增益因子。球形杀虫剂比来自Rps的更大。嗜酸性，这表明这两种不同的LH2以相反的方向吸附在带负电荷的硅胶壳上，并带有相反的电荷。观察到的这两种不同的LH2吸附到二氧化硅纳米粒子上时，BS50激发态寿命变化的差异进一步证明了这一点，其中B850的寿命充当纳米粒子诱导的蛋白质变形的内在报告物，蛋白质变形也取决于方向，由于B850环在LH2内部的不对称位置。

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