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首页> 外文期刊>The journal of physical chemistry, C. Nanomaterials and interfaces >Stability of the Disulfide Bond in Cystine Adsorbed on Silver and Gold Nanoparticles As Evidenced by SERS Data
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Stability of the Disulfide Bond in Cystine Adsorbed on Silver and Gold Nanoparticles As Evidenced by SERS Data

机译：SERS数据证明，银和金纳米粒子吸附的胱氨酸中二硫键的稳定性

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摘要

In relation with the difficulties encountered in previous works concerning the preservation of the S~S linkage in cystine (Cys-Cys dimer) on Ag nanoparticles (NPs), we present here a systematic investigation on both cysteine and cystine as a function of various parameters governing the preparation of metal substrates. Surface-enhanced Raman scattering (SERS) was used as a probe for analyzing (i) the integrity of the disulfide bonds of the adsorbed dimers, (ii) the influence of the metal nature, the reduction protocol as well as the Cys-Cys concentration on the adsorption, (iii) the terminal groups through which the interaction with metal surfaces take place, and (iv) the side chain conformation of the adsorbed molecules. From the whole set of experimental data collected in this work, it appears that large size Au NPs, prepared at low citrate concentration, can be considered as the most appropriate substrates for ensuring the integrity of disulfide linkages. Although Ag NPs prepared with hydroxylamine lead to the cleavage of dimers at low concentration, they have shown their adequacy to keep intact S~S bonds beyond a concentration threshold of ～200 μM. Based on the examination of the SERS data recorded as a function of dimer concentration, we can now assume that this effect is mainly due to the bidentate and monodentate binding of Cys-Cys dimers at low and high concentrations, respectively, facilitating or not their cleavage on Ag surfaces.
机译：关于先前工作中关于在银纳米颗粒（NPs）上保留胱氨酸（Cys-Cys二聚体）中S〜S键的困难，我们在这里对半胱氨酸和胱氨酸作为各种参数的函数进行了系统的研究。控制金属基材的制备。使用表面增强拉曼散射（SERS）作为探针来分析（i）吸附的二聚体的二硫键的完整性，（ii）金属性质，还原方案以及Cys-Cys浓度的影响关于吸附，（iii）与金属表面发生相互作用的末端基团，和（iv）被吸附分子的侧链构象。从这项工作中收集的全部实验数据来看，似乎可以将以低柠檬酸盐浓度制备的大尺寸金纳米颗粒视为确保二硫键完整性的最合适底物。尽管用羟胺制备的Ag NPs可以在低浓度下裂解二聚体，但它们已经显示出足以保持完整的S〜S键超过〜200μM阈值的能力。基于对记录为二聚体浓度函数的SERS数据的检查，我们现在可以假定这种作用主要是由于分别在低浓度和高浓度下Cys-Cys二聚体的二齿和单齿结合，促进或不裂解在银表面上。

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