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首页> 外文期刊>Cotton Reseach Journal >Expression of CrylAc and Cry2Ab in Bt Cotton Hybrids and their Toxicity to Helicoverpa armigera(Hub.) and Spodoptera litura (Fab.)
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Expression of CrylAc and Cry2Ab in Bt Cotton Hybrids and their Toxicity to Helicoverpa armigera(Hub.) and Spodoptera litura (Fab.)

机译：CrylAc和Cry2Ab在Bt棉杂交种中的表达及其对棉铃虫和斜纹夜蛾的毒性

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摘要

Experiment was conducted to determine the expression levels of Cry 1 Ac and Cry2Ab from transgenic cotton leaves of eighteen genotypes and mortality of Helicoverpa armigera (Hub.) and Spodoptera litura (Fab.) was studied on the same genotypes during kharif, 2011 -2012 at Department of Agricultural Entomology, VNMKV, Parbhani. The levels of insecticidal protein of Cry 1 Ac was found to be significantly highest (12.22 mug/g), in MRC 7351 BGII and UPLHH 2 Fusion Bt while in Dhanwan BGII (NCS 868) Cry2Abprotein content was 489.2 ig/g. The highest mortality (96.66 per cent) of Helicoverpa larvae was recorded in ACH 11 BG II and TCHH-4 BG II hybrids whereas the highest mortality (76.66 per cent) of Spodoptera larvae was observed in ACH 11 BG II, Kaveri Jackpot BG II and TCHH-4 BGII.
机译：进行实验以确定18种基因型的转基因棉花叶片中Cry 1 Ac和Cry2Ab的表达水平，并研究了2011年至2012年在哈里夫期间使用相同基因型的棉铃虫和斜纹夜蛾的死亡率。帕尔巴尼VNMKV农业昆虫学系。在MRC 7351 BGII和UPLHH 2 Fusion Bt中，发现Cry 1 Ac的杀虫蛋白水平最高（12.22大杯/克），而在Dhanwan BGII（NCS 868）中，Cry2Ab蛋白含量为489.2 ig / g。在ACH 11 BG II和TCHH-4 BG II杂种中，Helicoverpa幼虫的死亡率最高（96.66％），而在ACH 11 BG II，Kaveri Jackpot BG II和ACH 11 BG II中观察到的斜纹夜蛾幼虫的最高死亡率（76.66％）。 TCHH-4 BGII。

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