• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cotton Reseach Journal >Interspecific Hybridization between Gossypium hirsutum and G armourianum : Morphological and Molecular Characterization of Hybrids
【24h】

Interspecific Hybridization between Gossypium hirsutum and G armourianum : Morphological and Molecular Characterization of Hybrids

机译：陆地棉和军械菌种间杂交：杂种的形态和分子表征

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The interspecific hybrids G. hirsutum cv. LRA 5166 x G armourianum (Hybrid 1) and G. hirsutum cv. Sumangala x G. armourianum (Hybrid 2) were genotyped using Random Amplified Polymorphic DNA (RAPD) markers to confirm hybridity. For hybrid 1,11 of 30 random primers were informative and 15 of 34 primers were informative for hybrid 2. Based on the presence or absence of DNA bands in the female parent, triploid hybrid and male parent, the molecular markers could be classified into seven Types (I- VII). Results indicated that 48.2% and 55.5% of the RAPD markers had additivity among parents and off-spring in hybrid 1 and hybrid 2, respectively. However, 41.1% and 33.6% of parental markers (Type III, V and VII) were absent in hybrid 1 and hybrid 2, respectively. For both hybrids, 11% of the markers were unique (Type VI). The hybrids having Type IV markers (male specific) clearly showed that DNA based technology such as RAPD can be used to distinguish between true inter-specific hybrids of Gossypium and parental selfs. The morphological and cytological characters augmented with RAPD analysis indicated clear distinction between the parents and the resultant hybrids.
机译：种间杂种G.hirsutum cv。 LRA 5166 x G Armourianum（Hybrid 1）和G. hirsutum cv。使用随机扩增多态性DNA（RAPD）标记对Sumangala x G. armourianum（Hybrid 2）进行基因分型以确认杂交。对于杂种，在30个随机引物中有1,11个提供信息，而在34个引物中有15个对于杂种2提供了信息。根据雌性亲本，三倍体杂种和雄性亲本中是否存在DNA条带，分子标记可分为7种类型（I-VII）。结果表明，在杂种1和杂种2中，分别有48.2％和55.5％的RAPD标记具有亲本性。然而，杂种1和杂种2中分别没有41.1％和33.6％的亲本标记（类型III，V和VII）。对于这两个杂种来说，11％的标记是唯一的（VI型）。具有IV型标记（雄性特异性）的杂种清楚地表明，基于DNA的技术（例如RAPD）可用于区分棉属和亲本的真正种间杂种。通过RAPD分析增强的形态学和细胞学特征表明，亲本和所得杂种之间存在明显区别。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号