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首页> 外文期刊>Current Biology: CB >The C. elegans DYRK kinase MBK-2 marks oocyte proteins for degradation in response to meiotic maturation
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The C. elegans DYRK kinase MBK-2 marks oocyte proteins for degradation in response to meiotic maturation

机译：秀丽隐杆线虫DYRK激酶MBK-2标记卵母细胞蛋白响应减数分裂成熟而降解

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The oocyte-to-embryo transition transforms a differentiated germ cell into a totipotent zygote capable of somatic development. In C. elegans, several oocyte proteins, including the meiotic katanin subunit MEI-1 and the oocyte maturation protein OMA-1, must be degraded during this transition [1]. Degradation of MEI-1 and OMA-1 requires the dual-specificity YAK-1-related (DYRK) kinase MBK-2 [2-4]. Here, we demonstrate that MBK-2 directly phosphorylates MEI-1 and OMA-1 in vitro and that this activity is essential for degradation in vivo. Phosphorylation of MEI-1 by MBK-2 reaches maximal levels after the meiotic divisions, immediately preceding MEI-1 degradation. MEI-1 phosphorylation and degradation still occur in spe-9 eggs, which undergo meiotic maturation and exit in the absence of fertilization [5]. In contrast, MEI-1 phosphorylation and degradation are blocked in cell-cycle mutants that arrest during the meiotic divisions, and are accelerated in wee-1.3(RNAi) oocytes, which prematurely enter meiotic M phase (A. Golden, personal communication). A GFP:MBK-2 fusion relocalizes from the cortex to the cytoplasm during the meiotic divisions, and this relocalization also depends on cell-cycle progression. Our findings suggest that regulators of meiotic M phase activate a remodeling program, independently of fertilization, to prepare eggs for embryogenesis.
机译：卵母细胞到胚胎的过渡将分化的生殖细胞转化为能够进行体细胞发育的全能合子。在秀丽隐杆线虫中，几种卵母细胞蛋白，包括减数分裂的katanin亚基MEI-1和卵母细胞成熟蛋白OMA-1，必须在此过渡过程中降解[1]。 MEI-1和OMA-1的降解需要双重特异性YAK-1相关（DYRK）激酶MBK-2 [2-4]。在这里，我们证明了MBK-2在体外直接磷酸化MEI-1和OMA-1，并且该活性对于体内降解至关重要。 MBEI-2被MEI-1磷酸化后，在减数分裂分裂之后，即在MEI-1降解之前。 spe-9卵中仍会发生MEI-1磷酸化和降解，这些卵经历减数分裂成熟并在没有受精的情况下退出[5]。相反，MEI-1的磷酸化和降解在减数分裂分裂中停滞的细胞周期突变体中被阻止，并在过早进入减数分裂M期的wee-1.3（RNAi）卵母细胞中加速（A. Golden，个人交流）。 GFP：MBK-2融合蛋白在减数分裂分裂过程中从皮质重新定位到细胞质，这种重新定位还取决于细胞周期的进程。我们的发现表明，减数分裂M期的调控因子可独立于受精而激活重塑程序，从而为卵子的发生做好准备。

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