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Bio-efficacy and Mode of Action of Nano Pyridalyl Against Helicoverpa armigera

机译:纳米吡啶基对棉铃虫的生物功效和作用方式

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Suspension of pyridalyl nanocapsules was prepared in order to test whether the insecticidal efficacy of the molecule can be improved by nano sizing. The insecticidal activity of pyridalyl nanosuspension, in comparison to technical material and commercial formulation, was evaluated against larvae of Helicoverpa armigera by leaf dip method in vitro. The insecticidal activity of pyridalyl nanosuspension increased remarkably, with LC_(50) values of 40 ppm, in comparison to LC_(50) values of technical material (90 ppm) and commercial product (250 ppm). Bioassay results showed that nanoformulation is 2.26 and 6.25 times more toxic as stomach poison than the technical product and commercial formulation, respectively. For nanoformulation, technical materialand commercial formulation ratified our hypothesis. Mode of action study and validation of bioefficacy with first instar larvae further supported our arguments. The EC_(50) of pyridalyl and nano pyridalyl was 51.26 ppm and 16.74 ppm, respectively. EC_(50)of nano pyridalyl was thus threefold higher than pyridalyl. Pyridalyl nano-capsule suspension prepared using sodium alginate was sufficiently stable and performed more effectively than technical material and commercial product against cotton boll worm(Helicoverpa armigera), thus insecticide load to the environment can be reduced by using nanoformulations.
机译:制备吡啶二酮纳米胶囊的悬浮液是为了测试该分子的杀虫效力是否可以通过纳米施胶来提高。与工业材料和商业配方相比,吡虫啉纳米悬浮液的杀虫活性通过叶浸法体外评价了对棉铃虫的幼虫的杀虫活性。与工业材料(90 ppm)和商业产品(250 ppm)的LC_(50)值相比,吡啶基纳米悬浮液的杀虫活性显着提高,LC_(50)值为40 ppm。生物测定结果表明,纳米制剂的毒性是胃药的2.26倍和6.25倍,分别比工业产品和商业制剂高。对于纳米配方,技术材料和商业配方认可了我们的假设。作用模式研究和第一龄幼虫的生物功效验证进一步支持了我们的观点。吡啶基和纳米吡啶基的EC_(50)分别为51.26 ppm和16.74 ppm。因此,纳米吡咯的EC_(50)比吡咯高三倍。用海藻酸钠制备的吡啶基纳米胶囊混悬液比棉铃虫(Helicoverpa armigera)的工业原料和工业产品具有足够的稳定性和更有效的表现,因此可以通过使用纳米制剂减少杀虫剂对环境的负荷。

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