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Studies on Suitability of Cytoplasmic Genetic Male Sterility (CGMS) based Heterosis Breeding and its Causes in Upland Cotton (Gossypium hirsutum L.)

机译:基于细胞质遗传雄性不育(CGMS)的杂种优势育种及其在陆地棉上的研究

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Cotton is an often cross pollinated crop and possesses a considerable amount of heterosis. The hybrid seed production by conventional method is not possible being labour intensive. Several alternate methods for emasculation are available; however GMSand CMS systems are quite effective, because in rest of the methods there is always a chance of pistil damage and anthers are not completely eliminated. The success in development of CMS based hybrid largely depends on availability of stable male sterilecytoplasm and the effective restorer lines. However, the CMS system could not be exploited commercially because the G. harknessii cytoplasm and nuclear genes exhibited their deleterious effect on yield and related traits. Therefore, an investigation wastaken up in cotton {Gossypium hirsutum) to identify suitable male sterile line for the G. harknessii cytoplasm. Twenty two crosses were attempted between CMS lines and restorer lines. On the basis of per se performance for seed cotton yield, only one cross combination K34007 x CIR 70 (PS), for ginning out turn, 4 crosses viz., SH 2379 x CIR 23, F 1183 x CIR 23, LH 1134 x CIR 23 and RB 281 x CIR 38 and for fibre fineness, 11 crosses showed significant superiority over the conventional check hybrid CSHH198, which indicated that G harknessii cytoplasm may suppress the yield, but not for ginning out turn and fibre fineness. To know the causes of low yield in the CMS based cross combinations, these cross combinations were also selected for fertility restoration studies. On the basis of pollen abundancy and seed fertiUty restoration percentage, 8 crosses viz., LRA 5166 x CIR 32, LRA 5166 x CIR38, Jhorar x CIR 32, Jhorar x CIR 358, RB 281 x CIR 32, LH 1134 X CIR 32, F 1183 x CIR 23 and F 1183 x CIR26 had more than 90 per cent selfed seed. G hirsutum lines viz. CIR 23 and CIR 26 for CMS F1183, CIR 8 for CMS SH 2379, CIR 70 for CMS K 34007 were identified as new partial restorer lines and CIR 32 and CIR 38 for CMS hnes LRA 5166, Jhorar, RB 281 LH 1134 and F505 were potential restorer lines as these had shown maximum pollen abundancy as well as selfed seed setting percentage.
机译:棉花是经常交叉授粉的作物,并且具有相当大的杂种优势。通过常规方法的杂交种子生产不可能是劳动密集型的。有几种替代的去雄方法。但是,GMS和CMS系统非常有效,因为在其他方法中,总有雌蕊受损的可能,并且花药没有完全消除。基于CMS的杂种的成功开发很大程度上取决于稳定的雄性不育细胞质和有效的恢复系。然而,CMS系统不能被商业开发,因为哈克氏杆菌的细胞质和核基因对产量和相关性状表现出有害作用。因此,在棉花(棉(Gossypium hirsutum))中进行了调查,以鉴定出适合哈克氏杆菌细胞质的雄性不育系。尝试在CMS系和恢复系之间进行22次杂交。根据种子棉产量的本身表现,只有一个交叉组合K34007 x CIR 70(PS),用于轧花,四个交叉,即SH 2379 x CIR 23,F 1183 x CIR 23,LH 1134 x CIR 23和RB 281 x CIR 38以及11个杂交显示的纤维细度均优于常规格纹杂交CSHH198,这表明Garkarkii细胞质可以抑制产量,但不适用于轧制匝数和纤维细度。为了了解基于CMS的杂交组合低产的原因,还选择了这些杂交组合进行生育力恢复研究。根据花粉丰度和种子受精率,八个杂交即LRA 5166 x CIR 32,LRA 5166 x CIR38,Jhorar x CIR 32,Jhorar x CIR 358,RB 281 x CIR 32,LH 1134 X CIR 32, F 1183 x CIR 23和F 1183 x CIR26具有超过90%的自种种子。陆地棉线。 CMS F1183的CIR 23和CIR 26,CMS SH 2379的CIR 8,CMS K 34007的CIR 70被确定为新的部分恢复系,而CMS hnes LRA 5166,Jhorar,RB 281 LH 1134和F505的CIR 32和CIR 38被确定为潜在的恢复系,因为它们显示出最大的花粉丰度以及自种结实百分比。

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