Biomethane as a substitute for the fossil fuel natural gas offers a variety of options and applications for a sustainable energy supply, supporting the transition from fossil fuels to renewables. It can be produced by biomass thermochemical conversions (gasification) or by microbiological processes (anaerobic digestion). In both cases, the product gas requires upgrading prior the use. Gasification of biomass with all the subsequent steps to obtain biomethane high content is still at the research and demonstration stage [1]. On the other side, even though anaerobic digestion and biogas (raw biomethane) upgrading have been successfully demonstrated in various plants [2], there's room for further research in the context of process development and optimization. It has long been accepted the supremacy of two-stage anaerobic digestion (acidogenic stage separated from the methanogenic stages), reported as viable route to produce biohydrogen and biomethane from a wide range of organic materials [3,4]. However, further research is necessary to define the optimal process conditions to ensure higher performance.
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