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首页> 外文期刊>Virology >A chimeric virus created by DNA shuffling of the capsid genes of different subtypes of porcine circovirus type 2 (PCV2) in the backbone of the non-pathogenic PCV1 induces protective immunity against the predominant PCV2b and the emerging PCV2d in pigs
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A chimeric virus created by DNA shuffling of the capsid genes of different subtypes of porcine circovirus type 2 (PCV2) in the backbone of the non-pathogenic PCV1 induces protective immunity against the predominant PCV2b and the emerging PCV2d in pigs

机译：在非致病性PCV1的骨架中通过对猪圆环病毒2型（PCV2）的不同亚型的衣壳基因进行DNA改组而产生的嵌合病毒可诱导针对猪中PCV2b和PCV2d的保护性免疫

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Porcine circovirus type 2 (PCV2) is the primary causative agent of porcine circovirus-associated disease (PCVAD). Available commercial vaccines all target PCV2a subtype, although the circulating predominant subtype worldwide is PCV2b, and the emerging PCV2d subtype is also increasingly associated with PCVAD. Here we molecularly bred genetically-divergent strains representing PCV2a, PCV2b, PCV2c, PCV2d, and "divergent PCV2a" subtypes by DNA-shuffling of the capsid genes to produce a chimeric virus representing PCV2 global genetic diversity. When placed in the PCV2a backbone, one chimeric virus (PCV2-3c114) induced higher neutralizing antibody titers against different PCV2 subtypes. Subsequently, a candidate vaccine (PCV1-3cl14) was produced by cloning the shuffled 3cl14 capsid into the backbone of the non-pathogenic PCV1. A vaccine efficacy study revealed that chimeric virus PCV1-3cl14 induces protective immunity against challenge with PCV2b or PCV2d in pigs. The chimeric PCV1-3cl14 virus is a strong candidate for a novel vaccine in pigs infected with variable PCV2 strains. (C) 2016 Elsevier Inc. All rights reserved.
机译：猪圆环病毒2型（PCV2）是猪圆环病毒相关疾病（PCVAD）的主要病原体。尽管全球流行的主要亚型是PCV2b，但可用的商业疫苗都以PCV2a亚型为目标，而新兴的PCV2d亚型也越来越与PCVAD相关。在这里，我们通过衣壳基因的DNA改组，分子育成代表PCV2a，PCV2b，PCV2c，PCV2d和“差异PCV2a”亚型的遗传差异菌株，以产生代表PCV2全球遗传多样性的嵌合病毒。当置于PCV2a骨架中时，一种嵌合病毒（PCV2-3c114）诱导出针对不同PCV2亚型的更高的中和抗体效价。随后，通过将改组后的3cl14衣壳克隆到非病原性PCV1的骨架中来生产候选疫苗（PCV1-3cl14）。一项疫苗功效研究表明，嵌合病毒PCV1-3cl14可以诱导猪抵抗PCV2b或PCV2d攻击的保护性免疫。嵌合PCV1-3cl14病毒是感染可变PCV2株的猪中新型疫苗的强力候选者。 （C）2016 Elsevier Inc.保留所有权利。

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