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首页> 外文期刊>Vaccine >Protective antigens of Mycobacterium habana are distributed betweenperipheral and integral compartments of plasma membrane: a study inexperimental tuberculosis of mouse
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Protective antigens of Mycobacterium habana are distributed betweenperipheral and integral compartments of plasma membrane: a study inexperimental tuberculosis of mouse

机译：哈瓦那分枝杆菌的保护性抗原分布在质膜的周围和整体腔室之间：小鼠实验性肺结核研究

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摘要

Mycobacterium habana, a cultivable nonpathogenic mycobacterium provides appreciable resistance in mouse against M. tuberculosis infection. This study is aimed at identification anal characterization of protective antigens of M. habana. Protective potential of antigens of cell wall (CW), cell membrane (CM), cytosol (CS) and peripheral and integral compartments of the membrane fraction of M. habana was explored against experimental tuberculosis in mouse. Peripheral and integral membrane proteins were characterized by SDS-PAGE: and differential staining with silver and periodic acid. Results reveal that protective antigens are distributed in both peripheral and integral membrane compartments though such effect is dominant in the former. Polysaccharide staining showed that LAM, LM and PIMs have a preference for the detergent phase. Peripheral and integral compartments constitute, respectively, 68 and 31% of the total membrane protein. (C) 1999 Elsevier Science Ltd. All rights reserved. [References: 29]
机译：哈瓦那分枝杆菌是一种可培养的非致病性分枝杆菌，可在小鼠中对结核分枝杆菌感染提供明显的抗性。这项研究的目的是鉴定哈巴纳分枝杆菌保护性抗原的肛门特性。探索了哈瓦那分枝杆菌细胞壁（CW），细胞膜（CM），胞质溶胶（CS）以及外围和整合部分的抗原对小鼠实验性肺结核的保护潜力。外围和完整的膜蛋白通过SDS-PAGE进行表征：并用银和高碘酸进行差异染色。结果表明，保护性抗原既分布在外周膜区，又分布在整体膜区，尽管这种作用在前者中占主导地位。多糖染色表明，LAM，LM和PIM最喜欢去污剂相。外围和整体部分分别占总膜蛋白的68％和31％。 （C）1999 Elsevier ScienceLtd。保留所有权利。 [参考：29]

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