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首页> 外文期刊>Vaccine >Induction of humoral immune response against PfMSP-1(19) and PvMSP-1(19) using gold nanoparticles along with alum
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Induction of humoral immune response against PfMSP-1(19) and PvMSP-1(19) using gold nanoparticles along with alum

机译：使用金纳米粒子和明矾诱导针对PfMSP-1（19）和PvMSP-1（19）的体液免疫反应

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摘要

The C-terminal 19 kDa fragment of merozoite surface protein 1(MSP-1(19)) is an important vaccine candidate but a poor immunogen with available human compatible adjuvants like alum. We have investigated if Gold Nanoparticles (GNPs) based delivery system would enhance immune response to recombinant PfMSP-1(19) and PvMSP-1(19). We found that PfMSP-1(19)/PvMSP-1(19) coated on GNPs or in alum was poorly immunogenic in mice, a robust antibody response was observed when PfMSP-1(19)/PvMSP-1(19) coated GNPs were formulated with alum. PfMSP-1(19)/PvMSP-1(19) coated GNPs strongly recognized conformation specific monoclonal antibodies showing that capping on to GNPs did not compromise with conformational integrity of these antigens, which is critical for the generation of functional antibody response. Anti-PfMSP-1(19) antibodies raised with GNP-alum formulation recognized the native protein on parasite surface and showed invasion inhibition of Plasmodium falciparum parasite in an in vitro assay. Antigen coated GNPs with alum may be useful in the development of an alternate adjuvant formulation
机译：裂殖子表面蛋白1（MSP-1（19））的C端19 kDa片段是重要的候选疫苗，但免疫原性较弱，与可用的人类相容性佐剂如明矾一样。我们已经研究了基于金纳米颗粒（GNP）的传递系统是否会增强对重组PfMSP-1（19）和PvMSP-1（19）的免疫反应。我们发现涂在GNPs或明矾上的PfMSP-1（19）/ PvMSP-1（19）在小鼠中的免疫原性很差，当涂有PfMSP-1（19）/ PvMSP-1（19）的GNPs时观察到强大的抗体反应用明矾配制。 PfMSP-1（19）/ PvMSP-1（19）包被的GNP强烈识别构象特异性单克隆抗体，显示加帽到GNP不会损害这些抗原的构象完整性，这对于产生功能性抗体应答至关重要。用GNP-alum制剂培养的抗PfMSP-1（19）抗体可识别寄生虫表面上的天然蛋白质，并在体外试验中显示出对恶性疟原虫的入侵具有抑制作用。用明矾包被抗原的GNP可用于替代佐剂的开发

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