...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Vaccine >High antibody and cellular responses induced to HIV-1 clade C envelope following DNA vaccines delivered by electroporation
【24h】

High antibody and cellular responses induced to HIV-1 clade C envelope following DNA vaccines delivered by electroporation

机译：通过电穿孔递送DNA疫苗后，对HIV-1进化枝C包膜的高抗体和细胞应答

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: Clade C is the predominant HIV-1 strain infecting people in sub-Saharan Africa, India, and China and there is a critical need for a vaccine targeted to these areas. In this study we tested a DNA based vaccine that encodes the SIVgag, SIVpol and HIV-1 envelope clade C.Methods: Rhesus macaques were immunized by electroporation with the DNA plasmid encoding optimized SIVgag, SIVpol and an HIV-1 env clade C with or without the adjuvant RANTES. Animals were monitored for immune responses and challenged following the final immunization with 25 animal infectious doses (AID) of SHIV-1157ipd3N4.Results: We found that the vaccine induced high levels of antigen specific IFN-gamma producing effector cells and the capacity for CD4+ and CD8+ to proliferate upon antigen stimulation. Importantly, we found that the vaccine induced antibody titers as high as 1/4000. These antibodies were capable of neutralizing tier 1 HIV-1 viruses. Finally, when macaques were challenged with SHIV, viral loads were controlled in vaccinated groups.Conclusion: We conclude that immunization with a simian/human immunodeficiency virus DNA-based vaccine delivered by electroporation can induce cellular and humoral immune responses that are able to control viral replication
机译：背景：进化枝C是主要的HIV-1毒株，感染撒哈拉以南非洲，印度和中国的人们，因此迫切需要针对这些地区的疫苗。在这项研究中，我们测试了一种编码SIVgag，SIVpol和HIV-1包膜进化枝C的基于DNA的疫苗。方法：恒河猴猕猴通过电穿孔用编码优化的SIVgag，SIVpol和HIV-1进化枝C的DNA质粒进行免疫接种。没有佐剂RANTES。监测动物的免疫反应，并在最终免疫后用25种动物感染剂量（AID）的SHIV-1157ipd3N4攻击动物。结果：我们发现该疫苗诱导了高水平的抗原特异性IFN-γ产生效应细胞，并具有CD4 +和CD4 +的能力。 CD8 +在抗原刺激下增殖。重要的是，我们发现疫苗诱导的抗体效价高达1/4000。这些抗体能够中和1级HIV-1病毒。最后，当猕猴受到SHIV攻击时，接种疫苗组中的病毒载量得到控制。结论：我们得出结论，用电穿孔接种的猿猴/人免疫缺陷病毒基于DNA的疫苗进行免疫可以诱导能够控制病毒的细胞和体液免疫反应。复制

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号