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Alkyl hydroperoxide reductase: A candidate Helicobacter pylori vaccine

机译：烷基氢过氧化物还原酶：幽门螺杆菌候选疫苗

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Helicobacter pylori (H. pylori) is the most important etiological agent of chronic active gastritis, peptic ulcer disease and gastric cancer. The aim of this study was to evaluate the efficacy of alkyl hydroperoxide reductase (AhpC) and mannosylated AhpC (mAhpC) as candidate vaccines in the C57BL/6J mouse model of H. pylori infection. Recombinant AhpC was cloned, over-expressed and purified in an unmodified form and was also engineered to incorporate N and C-terminal mannose residues when expressed in the yeast Pichia pastoris. Mice were immunized systemically and mucosally with AhpC and systemically with mAhpC prior to challenge with H. pylori. Serum IgG responses to AhpC were determined and quantitative culture was used to determine the efficacy of vaccination strategies. Systemic prophylactic immunization with AhpC/alum and rnAhpC/alum conferred protection against infection in 55% and 77.3% of mice, respectively. Mucosal immunization with AhpC/cholera toxin did not protect against infection and elicited low levels of serum IgG in comparison with systemic immunization. These data support the use of AhpC as a potential vaccine candidate against H. pylori infection. (C) 2012 Elsevier Ltd. All rights reserved.
机译：幽门螺杆菌（H. pylori）是慢性活动性胃炎，消化性溃疡疾病和胃癌的最重要病因。这项研究的目的是评估烷基过氧化氢还原酶（AhpC）和甘露糖基化的AhpC（mAhpC）在幽门螺杆菌感染的C57BL / 6J小鼠模型中作为候选疫苗的功效。重组AhpC以未经修饰的形式克隆，过表达和纯化，并且经过工程改造，可在酵母毕赤酵母中表达时掺入N和C末端甘露糖残基。在用幽门螺杆菌攻击之前，用AhpC全身和粘膜免疫小鼠，并用mAhpC全身免疫小鼠。确定了血清对AhpC的IgG反应，并使用定量培养来确定疫苗接种策略的效力。用AhpC / alum和rnAhpC / alum进行的系统性预防性免疫分别在55％和77.3％的小鼠中提供了抗感染的保护作用。与全身免疫相比，用AhpC /霍乱毒素进行粘膜免疫不能预防感染，血清IgG水平低。这些数据支持将AhpC用作抗幽门螺杆菌感染的潜在候选疫苗。 （C）2012 Elsevier Ltd.保留所有权利。

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