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首页> 外文期刊>Vaccine >Evaluation of an improved rapid neutralizing antibody detection test (RAPINA) for qualitative and semiquantitative detection of rabies neutralizing antibody in humans and dogs
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Evaluation of an improved rapid neutralizing antibody detection test (RAPINA) for qualitative and semiquantitative detection of rabies neutralizing antibody in humans and dogs

机译：评估改进的快速中和抗体检测试验（RAPINA）用于人和狗中狂犬病中和抗体的定性和半定量检测

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Using the principle of immunochromatography, we previously developed a method called RAPINA (Rapid Neutralizing Antibody detection test) that can measure the level of rabies virus -neutralizing antibody (VNA) in serum samples [Shiota S. Mannen K, Matsumoto T, Yamada K, Yasui T, Takayama K. et al. Development and evaluation of a rapid neutralizing antibody test for rabies. J Virol Methods 2009;161:58-62]. RAPINA is faster, simpler, and easier to perform compared with a virus-neutralizing test or enzyme-linked immunosorbent assay (ELISA). The improved version of RAPINA has greater positive and negative predictive values corresponding to a VNA level of 0.5IU/mL, as recommended by the World Health Organization and the World Organization for Animal Health. To verify the efficacy of this improved method, serum samples were collected from humans and dogs before and after immunization against rabies and were tested in Japan, Sri Lanka, and Thailand. The results were compared between RAPINA and the true VNA levels measured by the Rapid Fluorescent Focus Inhibition Test (RFFIT). The improved RAPINA accurately predicted seropositivity for 182 of 183 seropositive human samples as assessed by RFFIT (99.5%) and for 138 of 140 RFFIT-negative human samples (98.6%). In dog serum samples, the positive and negative predictive values were 99.7% (345/355) and 95.6% (174/182), respectively. RAPINA was also used to estimate VNA levels in a semiquantitative manner by using serial dilution of serum samples. Our results show that RAPINA is an easy and rapid method for measuring VNA levels before and after immunization with the rabies vaccine and does not need a high skill level or sophisticated equipment. RAPINA can be used to monitor the success of preexposure prophylaxis in at-risk persons, vaccine coverage, and animal control. It can also be used in laboratories with modest facilities and where a large number of samples are screened. (C) 2012 Elsevier Ltd. All rights reserved.
机译：利用免疫色谱原理，我们先前开发了一种称为RAPINA（快速中和抗体检测测试）的方法，该方法可以测量血清样品中的狂犬病毒中和抗体（VNA）的水平[Shiota S. Mannen K，Matsumoto T，Yamada K， Yasui T，高山K.等。狂犬病快速中和抗体测试的开发和评估。 J Virol Methods 2009; 161：58-62]。与病毒中和测试或酶联免疫吸附测定（ELISA）相比，RAPINA更快，更简单，更容易执行。按照世界卫生组织和世界动物卫生组织的建议，改良版的RAPINA具有较高的阳性和阴性预测值，对应于0.5IU / mL的VNA水平。为了验证这种改进方法的有效性，在针对狂犬病免疫前后，从人和狗收集了血清样本，并在日本，斯里兰卡和泰国进行了测试。比较了RAPINA和通过快速荧光聚焦抑制测试（RFFIT）测量的真实VNA水平的结果。经RFFIT评估，改良的RAPINA可以准确预测183个血清阳性人样本中的182个（99.5％）和140个RFFIT阴性人样本中138个（98.6％）的血清阳性。在狗血清样本中，阳性和阴性预测值分别为99.7％（345/355）和95.6％（174/182）。通过使用血清样品的系列稀释，RAPINA还可以半定量方式估算VNA水平。我们的结果表明，RAPINA是一种在狂犬病疫苗免疫前后测量VNA水平的简便，快速的方法，不需要高技能水平或复杂的设备。 RAPINA可用于监测高危人群的暴露前预防，疫苗覆盖率和动物控制的成功。它也可以用在设施适中的实验室中，并在其中筛选大量样品。 （C）2012 Elsevier Ltd.保留所有权利。

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