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首页> 外文期刊>Vaccine >The immune enhancement of a novel soy lecithin/ beta -glucans based adjuvant on native Neospora caninum tachyzoite extract vaccine in mice.
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The immune enhancement of a novel soy lecithin/ beta -glucans based adjuvant on native Neospora caninum tachyzoite extract vaccine in mice.

机译：一种新型的基于大豆卵磷脂/β-葡聚糖的佐剂对小鼠天然新孢子虫速殖子提取物疫苗的免疫增强作用。

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Efficient, cost-effective and safe Th1-immunity-inducing vaccine formulations are paramount for achieving protection against Neospora caninum. In this study, a new adjuvant (Providean-AVECReg.) was used in the development of a N. caninum vaccine and evaluated in a mouse model. Soluble N. caninum tachyzoite native protein extract (sNcAg) was selected as vaccine antigen based on its capacity to activate production of pro-inflammatory cytokines on dendritic cells. Vaccines containing 4 and 0.4 micro g of sNcAg, and Providean-AVECReg., ISCOM-Matrix or aluminum hydroxide (Alum) were tested in BALB/c mice. While mice vaccinated with 4 micro g of sNcAg+Providean-AVECReg. developed specific antibodies shortly after the first dose, the rest of the high antigen payload formulations only induced seroconversion after the booster. Mice immunized with the high payload ISCOM vaccine (4 micro g sNcAg) or with either low or high payload Providean-AVECReg. formulations (0.4 micro g and 4 micro g sNcAg, respectively) elicited higher IgG2a than IgG1 serum levels, and IFN- gamma anamnestic responses with a Th1-cytokine biased profile. These animals had no histological signs of cerebral lesions and parasite burden assessed by quantitative real-time PCR was not detected. Vaccine preparations including Providean-AVECReg. as adjuvant limited N. canimum multiplication even with only a tenth of antigen payload compared to vaccines containing other adjuvants. Using adjuvants to specifically activate dendritic cells, combined with a careful antigen selection can enhance cellular responses to inert N. caninum vaccines.
机译：高效，经济高效且安全的Th1免疫诱导疫苗制剂对于获得针对犬新孢子虫的保护至关重要。在这项研究中，一种新的佐剂（ Providean -AVECReg。）用于开发 N。犬疫苗，并在小鼠模型中进行了评估。可溶性氮。基于犬类速殖子天然蛋白提取物（sNcAg）作为其激活树突状细胞促炎性细胞因子产生能力的疫苗抗原。在BALB / c小鼠中测试了含4和0.4微克sNcAg的疫苗以及 Providean -AVECReg。，ISCOM-Matrix或氢氧化铝（Alum）。小鼠接种了4微克sNcAg + Providean -AVECReg。在首次给药后不久就产生了特异性抗体，其余的高抗原有效载荷制剂仅在加强免疫后诱导血清转化。用高有效载荷ISCOM疫苗（4微克sNcAg）或低或高有效载荷 Providean -AVECReg免疫的小鼠。制剂（分别为0.4微克和4微克sNcAg）引起的IgG2a高于IgG1血清水平，并且IFN-γ记忆消除反应呈Th1细胞因子偏倚。这些动物没有脑部病变的组织学迹象，并且未检测到通过定量实时PCR评估的寄生虫负担。疫苗制剂包括 Providean -AVECReg。作为佐剂有限的。与仅含其他佐剂的疫苗相比，即使只有十分之一的抗原有效载荷也能进行最大倍增繁殖。使用佐剂特异性激活树突状细胞，再加上仔细的抗原选择，可以增强细胞对惰性N的反应。犬疫苗。

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