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首页> 外文期刊>Vaccine >Oral vaccination with vaccinia virus expressing the tick antigen subolesin inhibits tick feeding and transmission of Borrelia burgdorferi.
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Oral vaccination with vaccinia virus expressing the tick antigen subolesin inhibits tick feeding and transmission of Borrelia burgdorferi.

机译：表达壁虱抗原subolesin的牛痘病毒的口服疫苗抑制了伯氏疏螺旋体的壁虱摄食和传播。

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摘要

Immunization with the Ixodes scapularis protein, subolesin, has previously been shown to protect hosts against tick infestation and to decrease acquisition of Anaplsma marginale and Babesia bigemina. Here we report the efficacy of subolesin, a conserved tick protein that can act as a regulator of gene expression, expressed from vaccinia virus for use as an orally delivered reservoir - targeted vaccine for prevention of tick infestation and acquisition/transmission of Borrelia burgdorferi to its tick and mouse hosts. We cloned subolesin into vaccinia virus and showed that it is expressed from mammalian cells infected with the recombinant virus in vitro. We then vaccinated mice by oral gavage. A single dose of the vaccine was sufficient for mice to generate antibody response to subolesin. Vaccination with the subolesin expressing vaccinia virus inhibited tick infestation by 52% compared to control vaccination with vaccinia virus and reduced uptake of B. burgdorferi among the surviving ticks that fed to repletion by 34%. There was a reduction in transmission of B. burgdorferi to uninfected vaccinated mice of 40% compared to controls. These results suggest that subolesin has potential as a component of a reservoir targeted vaccine to decrease B. burgdorferi, Babesia and Anaplasma species infections in their natural hosts.
机译：以前已经证明，肩x突触小头蛋白亚油精的免疫作用可以保护宿主免受tick虫侵袭，并减少边缘无瓣念珠菌和大孢子虫的获得。在这里，我们报告Subolesin的功效，一种保守的tick蛋白，可以充当基因表达的调节剂，由痘苗病毒表达，用作口服贮藏库-靶向疫苗，可预防tick虫侵袭和获取/传播博氏疏螺旋体。刻度和鼠标主机。我们将亚油精蛋白克隆到牛痘病毒中，并表明它在体外被重组病毒感染的哺乳动物细胞中表达。然后我们通过管饲法给小鼠接种疫苗。单剂量疫苗足以使小鼠产生对亚油精的抗体反应。与接种牛痘病毒的对照疫苗相比，用表达油精蛋白的牛痘病毒疫苗进行的tick虫侵袭抑制了52％，并使存活下来的tick中的伯氏疏螺旋体的摄取减少了34％。与对照相比，B。burgdorferi向未感染疫苗的小鼠的传播减少了40％。这些结果表明，油精蛋白作为储库靶向疫苗的一部分具有潜力，可减少其天然宿主中的伯氏疏螺旋体，巴贝斯虫和无形体物种感染。

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