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首页> 外文期刊>Vaccine >Toxoplasma gondii immune mapped protein-1 (TgIMP1) is a novel vaccineTI Toxoplasma gondii immune mapped protein-1 (TgIMP1) is a novel vaccine candidate against toxoplasmosis
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Toxoplasma gondii immune mapped protein-1 (TgIMP1) is a novel vaccineTI Toxoplasma gondii immune mapped protein-1 (TgIMP1) is a novel vaccine candidate against toxoplasmosis

机译：弓形虫免疫定位蛋白1（TgIMP1）是一种新型疫苗TI弓形虫免疫定位蛋白1（TgIMP1）是一种新型抗弓形虫疫苗

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摘要

Immune mapped protein1 (IMP1) is a new protective protein in apicomplexan parasites, and exists in Toxoplasma gondii. In the present study, a DNA vaccine expressing IMP1 of T. gondii was constructed and the immune response induced in BALB/c mice was evaluated. The coding sequence of IMP1 was inserted into the eukaryotic expression vector pcDNA 3.1(+), resulting a recombinant plasmid pcDNA-IMP1, which was used to immunize BALB/c mice intramuscularly. After immunization, the immune response was evaluated using lymphoproliferative assay, and cytokine and antibody measurements. The mice were challenged with tachyzoites of the virulent T. gondii RH strain 14th day after the last immunization to observe the survival time. The results showed that the group immunized with pcDNA-IMP1 developed a high level of specific antibody responses against Escherichia coil expressed recombinant TgIMP1, with high IgG antibody titers, predominance of IgG2a production, a strong lymphoproliferative response, and significant levels of IFN-gamma, IL-2, IL-4 and IL-10 production compared with the control groups. These results demonstrate that pcDNA-IMP1 could elicit strong humoral and Th1 immune responses. Immunized mice showed a significantly (15.8 +/- 6 days) prolonged survival time compared with control mice, which died within 7 days of challenge infection. These results suggest that IMP1 is a promising vaccine candidate against toxoplasmosis
机译：免疫定位蛋白1（IMP1）是蚜虫复合体中的一种新型保护蛋白，存在于弓形虫中。在本研究中，构建了表达弓形虫IMP1的DNA疫苗，并评估了在BALB / c小鼠中诱导的免疫应答。将IMP1的编码序列插入真核表达载体pcDNA 3.1（+），得到重组质粒pcDNA-IMP1，用于肌肉内免疫BALB / c小鼠。免疫后，使用淋巴增生测定以及细胞因子和抗体测量评估免疫应答。在最后一次免疫后第14天，用强毒弓形虫RH株的速殖子攻击小鼠，观察其存活时间。结果表明，用pcDNA-IMP1免疫的组对埃希氏线圈表达的重组TgIMP1产生了高水平的特异性抗体反应，具有高IgG抗体滴度，IgG2a产生优势，强烈的淋巴增生反应以及显着水平的IFN-γ，与对照组相比IL-2，IL-4和IL-10的产生。这些结果表明pcDNA IMP1可以引起强烈的体液和Th1免疫反应。与对照小鼠相比，免疫小鼠表现出明显的（15.8 +/- 6天）延长的存活时间，而对照小鼠在感染后7天内死亡。这些结果表明，IMP1是抗弓形虫病的有前途的候选疫苗

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