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首页> 外文期刊>Vaccine >Protective efficacy against Chlamydophila psittaci by oral immunization based on transgenic rice expressing MOMP in mice
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Protective efficacy against Chlamydophila psittaci by oral immunization based on transgenic rice expressing MOMP in mice

机译：转基因水稻表达MOMP的小鼠口服免疫对鹦鹉热衣原体的保护作用

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摘要

Avian chlamydiosis is caused by Chlamydophila psittaci (Cp. psittaci) and major outer membrane protein (MOMP) of Cp. psittaci is an excellent vaccine candidate. In this study, the MOMP gene was expressed in rice callus by the Agrobacterium tumefaciensvector. The production of protein in transgenic rice seeds was confirmed and quantified by Western-blot and ELISA, the results demonstrating that the antigen was expressed stably. The transgenic rice seeds expressing the MOMP protein were administered by the oral route to BALB/c mice, which developed MOMP-specific serum IgG and fecal IgA antibodies and a splenocyte MOMP-specific proliferative response and significant levels of IFN-gamma, IL-2, IL-4, IL-5 and TGF-beta production. Immunization with MOMPtransgenic seeds induced partial protection (50%) against a lethal challenge with the highly virulent Cp. psittaci 6BC strain. Lung function after challenge was less affected compared non-MOMP immunized animals. The results demonstrate the feasibility ofusing transgenic rice seeds as an oral vaccine to generate protective immunity and reduce the lung lesions in mice against virulent Cp. psittaci 6BC strain. This finding has implications for further development of an oral vaccine against avian chlamydiosis.
机译：禽衣原体病是由鹦鹉热衣原体（Cp。psittaci）和主要的外膜蛋白（MOMP）引起的。 psittaci是出色的疫苗候选者。在这项研究中，MOMP基因通过根癌土壤杆菌载体在水稻愈伤组织中表达。 Western blot和ELISA证实并定量了转基因水稻种子中蛋白质的产生，结果表明该抗原稳定表达。通过口服途径将表达MOMP蛋白的转基因水稻种子给予BALB / c小鼠，BALB / c小鼠产生了MOMP特异性血清IgG和粪便IgA抗体以及脾细胞MOMP特异性增殖反应和显着水平的IFN-γ，IL-2 ，IL-4，IL-5和TGF-β的产生。用MOMP转基因种子免疫诱导了针对高毒性Cp的致命性攻击的部分保护（50％）。 psittaci 6BC菌株。与非MOMP免疫动物相比，攻击后的肺功能受到的影响较小。结果证明了将转基因水稻种子用作口服疫苗以产生保护性免疫力并减少小鼠针对强毒Cp的肺部损伤的可行性。 psittaci 6BC菌株。这一发现对进一步开发抗禽衣原体病的口服疫苗具有重要意义。

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