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首页> 外文期刊>Vaccine >SPI1 defective mutants of Salmonella enterica induce cross-protective immunity in chickens against challenge with serovars Typhimurium and Enteritidis.
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SPI1 defective mutants of Salmonella enterica induce cross-protective immunity in chickens against challenge with serovars Typhimurium and Enteritidis.

机译：肠道沙门氏菌的SPI1缺陷型突变体可诱导鸡对血清鼠伤寒和肠炎沙门氏菌的交叉保护免疫。

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摘要

In this study we were interested in the serovar cross-protection potential of Salmonella Pathogenicity Island 1 (SPI1) attenuated vaccine strains of Salmonella enterica serovars Enteritidis and Typhimurium and immune response of vaccinated and naive chickens to Salmonella infection. The immune response was characterized by real time PCR quantifying transcripts of interleukins IL1 beta , IL17, IL22, interferon gamma (IFN gamma ), inducible NO synthase (iNOS), immunoglobulins IgM, IgA, IgY and Ig light chain, and six genes of acute phase response including avidin, serum amyloid A, extracellular fatty acid-binding protein (Ex-FABP), immune responsive gene 1, chemokine AH221 and trappin-6. Vaccination with SPI1 mutants of both serovars protected chickens against Salmonella infection, independent of the serovar used for the challenge and the time post infection. However, expressions of all interleukins, iNOS and Ex-FABP showed that protection against homologous serovars was significantly higher than against heterologous serovars after intravenous challenge at 4 days post infection. The vaccination with a mixture of S. Enteritidis and S. Typhimurium SPI1 mutants induced an intermediate protection against challenge with both serovars, i.e. the mixed vaccine provided an additional protective effect when compared with the chickens vaccinated with a vaccine formed by only a single Salmonella serovar.
机译：在这项研究中，我们对沙门氏菌致病岛1（SPI1）减毒肠炎沙门氏菌和鼠伤寒沙门氏菌和鼠伤寒沙门氏菌的血清交叉保护潜力以及接种过的和初次接种的鸡对沙门氏菌的免疫应答感兴趣。通过实时PCR定量白介素IL1β，IL17，IL22，干扰素γ（IFNγ），诱导型一氧化氮合酶（iNOS），免疫球蛋白IgM，IgA，IgY和Ig轻链的转录本以及六个急性基因来表征免疫应答阶段反应包括抗生物素蛋白，血清淀粉样蛋白A，细胞外脂肪酸结合蛋白（Ex-FABP），免疫反应基因1，趋化因子AH221和trappin-6。两种血清型的SPI1突变体的疫苗接种均能保护鸡免受沙门氏菌感染，而不受用于攻击的血清型和感染后时间的影响。但是，所有白介素，iNOS和Ex-FABP的表达均表明，感染后4天静脉攻击后，对同源血清的保护作用明显高于对异源血清的保护作用。用肠炎沙门氏菌和鼠伤寒沙门氏菌SPI1突变体的混合物进行疫苗接种可诱导针对两种血清病毒的中间保护作用，即与仅接种单一沙门氏菌血清疫苗的鸡相比，混合疫苗可提供额外的保护作用。

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