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首页> 外文期刊>Vaccine >Gene delivery to dendritic cells by orally administered recombinant Saccharomyces cerevisiae in mice.
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Gene delivery to dendritic cells by orally administered recombinant Saccharomyces cerevisiae in mice.

机译：通过在小鼠中口服重组酿酒酵母将基因递送至树突状细胞。

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摘要

DNA vaccination has caught the attention of many for triggering humoral as well as cellular immune responses. And delivering DNA into the antigen presenting cells (APCs) in order to induce efficient immunoresponse has become the backbone of this field. It has been confirmed that Saccharomyces cerevisiae, though non-pathogenic, is being engulfed by the dendritic cells and macrophages and delivers not only proteins, but also DNA materials (already confirmed in vitro). In this research, S. cerevisiae is used to deliver green fluorescent protein (GFP) reporter gene controlled under cytomegalovirus (CMV) promoter in living organism (mice). The recombinant yeast, transfected with the plasmid containing the GFP gene, was heat killed and orally administered to mice. After 60 h of yeast administration, mice were sacrificed and intestine was separated, washed and frozen in liquid nitrogen. Tissues were cut at the size of 10 micro m using Cryostat machine, and GFP expression was successfully detected under a fluorescence microscope. After 45 days Western blot was able to detect GFP antibody in the blood of mice. These results imply that S. cerevisiae, being non-pathogenic, cheap, and easy to culture could be a good candidate to deliver DNA materials to the immune cells for vaccination.Digital Object Identifier http://dx.doi.org/10.1016/j.vaccine.2012.11.048
机译：DNA疫苗接种引起了许多人的关注，它们引发体液和细胞免疫反应。为了诱导有效的免疫反应而将DNA递送到抗原呈递细胞（APC）中已经成为该领域的骨干。已经证实，酿酒酵母尽管是非致病性的，但仍被树突状细胞和巨噬细胞吞噬，不仅传递蛋白质，而且传递DNA材料（已在体外得到证实）。在这项研究中，酿酒酵母用于在活生物体（小鼠）中传递受巨细胞病毒（CMV）启动子控制的绿色荧光蛋白（GFP）报告基因。用含有GFP基因的质粒转染的重组酵母被热杀死并口服给予小鼠。施用酵母60小时后，处死小鼠，分离肠，洗涤并在液氮中冷冻。使用冷冻恒温器将组织切成10微米的大小，并且在荧光显微镜下成功检测到GFP表达。 45天后，Western blot能够检测小鼠血液中的GFP抗体。这些结果表明，非致病性，廉价且易于培养的酿酒酵母可能是将DNA材料输送至免疫细胞进行疫苗接种的良好候选者。数字对象标识符http://dx.doi.org/10.1016/ j.vaccine.2012.11.048

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