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首页> 外文期刊>Vaccine >Plant heat shock protein 90 as carrier-adjuvant for immunization against a reporter antigen.
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Plant heat shock protein 90 as carrier-adjuvant for immunization against a reporter antigen.

机译：将植物热激蛋白90作为载体佐剂用于针对报告抗原的免疫接种。

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Here, we evaluated the modulation of the immune response induced by Hsp90 of Nicotiana benthamiana (NbHsp90.3) against the Maltose Binding Protein (MBP) as a reporter antigen. Equimolar quantities of recombinant proteins were administered in mice as follows: MBP alone (MBP group), a mixture of MBP and rNbHsp90.3 (MBP+rNbHsp90.3 group) and the fusion of MBP to rNbHsp90.3 (MBP-rNbHsp90.3 group). The covalent linkage between NbHsp90.3 and MBP to bring a fusion protein was essential to induce the strong specific antibody response with predominance of IgG2a. Eighty-four days after the first immunization, splenocyte proliferation from MBP-rNbHsp90.3-immunized mice was consistently higher than that from MBP and MBP+rNbHsp90.3 groups. In addition, splenocytes from MBP-rNbHsp90.3 immunized mice produced higher levels of IFN- gamma than controls. Finally, both formulations with rNbHsp90.3 significantly enhanced the MHC class I expression levels, but only rNbHsp90.3 covalent bound to MBP induced a specific cellular immune response against MBP measured as increased percentage of CD8+ T cells. Taken together, these results suggest that plant HSP90s could be incorporated as adjuvants in vaccines that require the generation of a Th1 response along with a CD8 cytotoxic cell response to confer immunity.
机译：在这里，我们评估了由本氏烟草的Hsp90（NbHsp90.3）诱导的针对麦芽糖结合蛋白（MBP）作为报告抗原的免疫应答的调节。将等摩尔量的重组蛋白按以下方式在小鼠中施用：单独的MBP（MBP组），MBP和rNbHsp90.3的混合物（MBP + rNbHsp90.3组）以及MBP与rNbHsp90.3的融合体（MBP-rNbHsp90.3）组）。 NbHsp90.3和MBP之间的共价键以产生融合蛋白对于诱导以IgG2a为主的强特异性抗体应答至关重要。第一次免疫后第84天，MBP-rNbHsp90.3免疫小鼠的脾细胞增殖始终高于MBP和MBP + rNbHsp90.3组。此外，来自MBP-rNbHsp90.3免疫小鼠的脾细胞产生的IFN-γ水平高于对照组。最后，两种具有rNbHsp90.3的制剂均显着提高了I类MHC的表达水平，但只有与MBP结合的rNbHsp90.3共价诱导了针对MBP的特异性细胞免疫应答，以CD8 ​​ + T细胞百分比的增加来衡量。综上所述，这些结果表明植物HSP90可以作为佐剂掺入需要产生Th1应答以及CD8细胞毒性细胞应答以赋予免疫力的疫苗中。

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