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首页> 外文期刊>Vaccine >Synthetic B- and T-cell epitope peptides of porcine reproductive and respiratory syndrome virus with Gp96 as adjuvant induced humoral and cell-mediated immunity.
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Synthetic B- and T-cell epitope peptides of porcine reproductive and respiratory syndrome virus with Gp96 as adjuvant induced humoral and cell-mediated immunity.

机译：以Gp96为佐剂的猪繁殖与呼吸综合征病毒的合成B细胞和T细胞表位肽诱导体液和细胞介导的免疫。

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摘要

Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) has recently caused huge economic losses in the pig industry worldwide. Commercial vaccines, including inactivated vaccines and attenuated live vaccines, are available but fail to provide sustainable protection, especially against genetically heterologous strains. Thus several approaches have been used to develop more effective PRRSV vaccines and/or immune modulators to accelerate and magnify immune responses to PRRSV vaccines. Heat shock protein Gp96 is one such modulator that enhances both the innate and adaptive immune responses. In the present study, two B-cell epitopes and seven T-cell epitopes from PRRSV and a Pan DR T-helper cell epitope were synthesized and mixed with the N-terminal 22-355 aa of Gp96 (Gp96N) as an adjuvant, and immune responses were evaluated. Our results show that Gp96N activated PRRSV-specific humoral immune responses elicited by BCE-peptides and promoted the PRRSV-specific cellular immunity induced by TCE-peptides. Moreover, higher levels of IL-12 and TNF- alpha and lower levels of IL-4 and IL-10 were observed in the serum of Gp96N-vaccinated piglets compared to piglets immunized with no Gp96N, displaying a predominant Th1 type of immune response induced by Gp96N. Following challenge with the virulent HP-PRRSV isolate JXwn06, piglets vaccinated with the mixture of peptides and Gp96N presented with milder clinical symptoms, lower viremia, and less pathological lesions in their lungs, however, this vaccine could not provide lasting and effective protection against HP-PRRSV infection. These data provide important bases for the development of PRRSV epitope-based synthetic peptide vaccines combined with Gp96N as attractive immunomodulators in swine.
机译：高致病性猪繁殖与呼吸综合征病毒（HP-PRRSV）最近在全世界的养猪业中造成了巨大的经济损失。可以买到商业疫苗，包括灭活疫苗和减毒活疫苗，但不能提供可持续的保护，尤其是针对遗传异源菌株的保护。因此，已经使用几种方法来开发更有效的PRRSV疫苗和/或免疫调节剂以加速和放大对PRRSV疫苗的免疫应答。热休克蛋白Gp96是一种这样的调节剂，其增强先天和适应性免疫应答。在本研究中，合成了来自PRRSV的两个B细胞表位和七个T细胞表位以及Pan DR T辅助细胞表位，并与Gp96（Gp96N）的N端22-355aa混合，并作为佐剂。评估免疫反应。我们的结果表明，Gp96N激活了BCE肽引起的PRRSV特异性体液免疫反应，并促进了TCE肽诱导的PRRSV特异性细胞免疫。此外，与未接种Gp96N的仔猪相比，接种Gp96N的仔猪血清中观察到更高水平的IL-12和TNF-α以及更低水平的IL-4和IL-10，显示出主要的Th1型免疫应答由Gp96N。用强毒的HP-PRRSV分离株JXwn06攻击后，接种了肽和Gp96N混合物的仔猪表现出较轻的临床症状，较低的病毒血症和较少的肺部病理性病变，但是，这种疫苗无法提供持久有效的针对HP的保护-PRRSV感染。这些数据为开发基于PRRSV表位的合成肽疫苗与Gp96N结合作为猪中诱人的免疫调节剂提供了重要基础。

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