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首页> 外文期刊>Vaccine >A triple gene mutant of BoHV-1 administered intranasally is significantly more efficacious than a BoHV-1 glycoprotein E-deleted virus against a virulent BoHV-1 challenge
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A triple gene mutant of BoHV-1 administered intranasally is significantly more efficacious than a BoHV-1 glycoprotein E-deleted virus against a virulent BoHV-1 challenge

机译：经鼻内施用的BoHV-1的三基因突变体比BoHV-1糖蛋白E缺失病毒对有毒的BoHV-1攻击的功效明显更高

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Bovine herpesvirus 1 (BoHV-1) causes respiratory infections and abortions in cattle, and is an important component of bovine respiratory disease complex, which causes a considerable economic loss worldwide. Several efforts have been made to produce safer and more effective vaccines. One of these vaccines is a glycoprotein E (gE)-deleted marker vaccine which is currently mandated for use in EU countries. In the present study, we have constructed a three-gene-mutated BoHV-1 vaccine virus (U(L)49.5 luminal domain residues 30-32 and cytoplasmic tail residues 80-96 deleted, gE cytoplasmic tail- and entire Us9-deleted) and compared its protective vaccine efficacy in calves after intranasal vaccination with that of a gE-deleted virus. Following vaccination, both the triple mutant and gE-deleted vaccine virus replicated well in the nasal epithelium of the calves. The vaccinated calves did not show any clinical signs. Four weeks post-vaccination, the animals were challenged intranasally with a virulent BoHV-1 wild-type virus. Based on clinical signs, both the gE-deleted and triple mutant group were protected equally against the virulent BoHV-1 challenge. However, based on the quantity and duration of nasal viral shedding, virus neutralizing antibody and cellular immune responses, the triple mutant virus vaccine induced a significantly better protective immune response than the gE-deleted virus vaccine. Notably, after the virulent BoHV-1 challenge, the triple mutant virus vaccinated group cleared the challenge virus three days earlier than the BoHV-1 gE-deleted virus vaccinated group
机译：牛疱疹病毒1（BoHV-1）引起牛的呼吸道感染和流产，并且是牛呼吸道疾病综合症的重要组成部分，在世界范围内造成相当大的经济损失。为了生产更安全，更有效的疫苗，已经做出了一些努力。这些疫苗之一是缺失糖蛋白E（gE）的标记疫苗，目前已强制在欧盟国家/地区使用。在本研究中，我们构建了三基因突变的BoHV-1疫苗病毒（U（L）49.5腔结构域残基30-32和胞质尾部残基80-96缺失，gE胞质尾部和整个Us9缺失）并比较了鼻内疫苗接种后与gE缺失病毒对牛犊的保护性疫苗功效。接种疫苗后，三联突变体和gE缺失疫苗病毒均在小牛的鼻上皮中复制良好。接种的小牛没有任何临床症状。疫苗接种后四周，用强毒BoHV-1野生型病毒对动物进行鼻内攻击。根据临床体征，缺失gE的三突变体组和三突变体组均受到相同的保护，以抵抗有力的BoHV-1攻击。但是，根据鼻病毒脱落的数量和持续时间，病毒中和抗体以及细胞免疫应答，三元突变病毒疫苗诱导的保护性免疫应答明显优于缺失gE的病毒疫苗。值得注意的是，在强毒的BoHV-1攻击后，接种三联突变病毒的组比BoHV-1 gE缺失病毒接种组提前三天清除了攻击病毒

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