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首页> 外文期刊>Vaccine >A single dose of whole inactivated H7N9 influenza vaccine confers protection from severe disease but not infection in ferrets
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A single dose of whole inactivated H7N9 influenza vaccine confers protection from severe disease but not infection in ferrets

机译：单一剂量的全灭活H7N9流感疫苗可预防严重疾病，但不能感染雪貂

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The H7N9 influenza virus caused significant mortality and morbidity in infected humans during an outbreak in China in 2013 stimulating vaccine development efforts. As previous H7-based vaccines have been poorly immunogenic in humans we sought to determine the immunogenic and protective properties of an inactivated whole virus vaccine derived from a 2013 H7N9 virus in ferrets. As whole virus vaccine preparations have been shown to be more immunogenic in humans, but less likely to be used, than split or surface antigen formulations, we vaccinated ferrets with a single dose of 15, 30, or 50 mu g of the vaccine and subsequently challenged with wild-type A/Anhui/1/2013 (H7N9) either by direct instillation or by contact with infected animals. Although ferrets vaccinated with higher doses of vaccine had higher serum hemagglutinin inhibition (HI) titers, the titers were still low. During subsequent instillation challenge, however, ferrets vaccinated with 50 mu g of vaccine showed no illness and shed significantly less virus than mock vaccinated controls. All vaccinated ferrets had lower virus loads in their lungs as compared to controls. In a separate study where unvaccinated-infected ferrets were placed in the same cage with vaccinated-uninfected ferrets, vaccination did not prevent infection in the contact ferrets, although they showed a trend of lower viral load. Overall, we conclude that inactivated whole-virus H7N9 vaccine was able to reduce the severity of infection and viral load, despite the lack of hemagglutinin-inhibiting antibodies
机译：在2013年的中国爆发大流行中，H7N9流感病毒在感染人类中造成了巨大的死亡率和发病率，刺激了疫苗开发工作。由于以前的基于H7的疫苗在人体中的免疫原性较差，因此我们试图确定源自2013 H7N9病毒的雪貂灭活的全病毒灭活疫苗的免疫原性和保护性。由于全病毒疫苗制剂已显示对人类具有更高的免疫原性，但与分裂或表面抗原制剂相比具有更低的免疫原性，因此我们给雪貂接种了15、30或50μg单剂量疫苗，随后进行了接种通过直接滴注或接触感染的动物而受到野生型A / Anhui / 1/2013（H7N9）的攻击。尽管用较高剂量疫苗接种的雪貂的血清血凝素抑制（HI）滴度较高，但滴度仍然很低。然而，在随后的滴注攻击过程中，与模拟疫苗接种的对照组相比，接种了50微克疫苗的雪貂没有出现任何疾病，并且所散发的病毒明显减少。与对照相比，所有接种过的雪貂的肺部病毒载量均较低。在另一项研究中，将未接种疫苗的雪貂与未接种疫苗的雪貂放在同一个笼子里，接种疫苗并不能预防接触雪貂的感染，尽管它们显示出病毒载量降低的趋势。总的来说，我们得出的结论是，尽管缺乏抑制血凝素的抗体，但灭活的全病毒H7N9疫苗能够降低感染的严重程度和病毒载量

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