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首页> 外文期刊>Vaccine >Focused human gene expression profiling using dual-color reverse transcriptase multiplex ligation-dependent probe amplification
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Focused human gene expression profiling using dual-color reverse transcriptase multiplex ligation-dependent probe amplification

机译：使用双色逆转录酶多重连接依赖探针扩增的聚焦人类基因表达谱

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摘要

To investigate the human immune response to newly developed or existing vaccines, or during infection/disease on a population scale, we have recently developed a dual-color Reverse Transcriptase Multiplex Ligation-dependent Probe Amplification (dcRT-MLPA) assay, which can rapidly profile mRNA expression of multiple host genes. dcRT-MLPA has a dynamic range and sensitivity comparable to real-time QPCR and RNA-Sequencing. Since this assay is high-throughput, it is an exceptionally suitable technique for monitoring host biomarkers in semi-large scale human cohorts, such as cross sectional studies with multiple groups, or longitudinal studies with multiple time points. Multicomponent host biomarker signatures with excellent predictive values can easily be identified using lasso regression analysis, while exploring additional data adjustment methods like RUV-2 may further optimize the identification of informative host biomarker signatures. dcRT-MLPA also allows comparisons of gene expression patterns across different human populations to explore the impact of geographical diversity on for example vaccine induced responses. The use of dcRT-MLPA is not limited to peripheral blood but can be adapted to analyze host biomarkers derived from any tissue or body fluids, further demonstrating the versatility of the dcRT-MLPA platform. Several examples will be given and discussed. (C) 2015 Elsevier Ltd. All rights reserved.
机译：为了研究人类对新开发或现有疫苗的免疫应答，或在人群规模的感染/疾病期间的免疫应答，我们最近开发了一种双色逆转录酶多路结扎依赖性探针扩增（dcRT-MLPA）检测方法，可以快速分析多个宿主基因的mRNA表达。 dcRT-MLPA的动态范围和灵敏度与实时QPCR和RNA测序相当。由于此测定法的通量很高，因此它是用于监控半大规模人类队列中宿主生物标志物的非常合适的技术，例如多组横断面研究或多时间点纵向研究。使用套索回归分析可以轻松识别具有出色预测值的多组分宿主生物标志物标记，同时探索诸如RUV-2之类的其他数据调整方法可以进一步优化信息丰富的宿主生物标志物标记的识别。 dcRT-MLPA还允许比较不同人群的基因表达模式，以探索地理多样性对疫苗诱导反应的影响。 dcRT-MLPA的使用不仅限于外周血，还可以用于分析源自任何组织或体液的宿主生物标志物，从而进一步证明了dcRT-MLPA平台的多功能性。将给出并讨论几个示例。 （C）2015 Elsevier Ltd.保留所有权利。

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