The site of administration influences both the type and the magnitude of the immune response induced by DNA vaccine electroporation

Vandermeulen Gaelle; Vanvarenberg Kevin; De Beuckelaer Ans; De Koker Stefaan; Lambricht Laure; Uyttenhove Catherine; Reschner Anca; Vanderplasschen Alain; Grooten Johan; Preat Veronique

首页> 外文期刊>Vaccine >The site of administration influences both the type and the magnitude of the immune response induced by DNA vaccine electroporation

【24h】

The site of administration influences both the type and the magnitude of the immune response induced by DNA vaccine electroporation

机译：给药部位会影响DNA疫苗电穿孔诱导的免疫反应的类型和强度

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

We investigated the influence of the site of administration of DNA vaccine on the induced immune response. DNA vaccines were administered by electroporation at three different sites: tibial cranial muscle, abdominal skin and ear pinna. Aiming to draw general conclusions about DNA vaccine delivery, we successively used several plasmids encoding either luciferase and ovalbumin as models or gp160 and PIA as vaccines against HIV and P815 mastocytoma, respectively. Low levels and duration of luciferase transgene expression were observed after electroporation of the abdominal skin, partly explaining its lower immunogenic performance as compared to the other sites of administration. Analyses of OT-I CD8+ and OT-II CD4+ T cell responses highlighted the differential impact of the delivery site on the elicited immune response. Muscle electroporation induced the strongest humoral immune response and both muscle and ear pinna sites induced cellular immunity against gp160. Ear pinna delivery generated the highest level of CTL responses against PIA but electrop oration of muscle and ear pinna were equally efficient in delaying P815 growth and improving mice survival. The present study demonstrated that the site of administration is a key factor to be tested in the development of DNA vaccine.(C) 2015 Elsevier Ltd. All rights reserved.

机译：我们调查了DNA疫苗给药部位对诱导的免疫反应的影响。 DNA疫苗是通过电穿孔在三个不同部位施用的：胫骨颅肌，腹部皮肤和耳廓。为了得出有关DNA疫苗递送的一般结论，我们先后使用了编码荧光素酶和卵清蛋白的几种质粒作为模型，或分别使用gp160和PIA作为抗HIV和P815肥大细胞瘤的疫苗。在腹部皮肤电穿孔后观察到萤光素酶转基因表达的水平低且持续时间长，部分解释了与其他给药部位相比其免疫原性较低。 OT-I CD8 +和OT-II CD4 + T细胞反应的分析突显了递送部位对引发的免疫反应的不同影响。肌肉电穿孔诱导最强的体液免疫反应，而肌肉和耳廓部位均诱导针对gp160的细胞免疫。耳廓递送对PIA产生最高水平的CTL反应，但是肌肉和耳廓的电穿孔术在延迟P815生长和改善小鼠存活方面同样有效。本研究表明，给药部位是DNA疫苗开发中要测试的关键因素。（C）2015 Elsevier Ltd.保留所有权利。

著录项

来源
《Vaccine》 |2015年第28期|共7页
作者
Vandermeulen Gaelle; Vanvarenberg Kevin; De Beuckelaer Ans; De Koker Stefaan; Lambricht Laure; Uyttenhove Catherine; Reschner Anca; Vanderplasschen Alain; Grooten Johan; Preat Veronique;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类
关键词
DNA vaccine; Electroporation; Delivery site; Tumor vaccine; HIV vaccine;

机译：DNA疫苗;电气化;交货地点;肿瘤疫苗;HIV疫苗;

相似文献

外文文献
中文文献
专利

1. The site of administration influences both the type and the magnitude of the immune response induced by DNA vaccine electroporation [J] . Vandermeulen Gaelle, Vanvarenberg Kevin, De Beuckelaer Ans, Vaccine . 2015,第28期

机译：给药部位会影响DNA疫苗电穿孔诱导的免疫反应的类型和强度
2. Administration of HPV DNA vaccine via electroporation elicits the strongest CD8+ T cell immune responses compared to intramuscular injection and intradermal gene gun delivery. [J] . Best S. R., Peng S. W., Juang ChiMou, Vaccine . 2009,第40期

机译：与肌肉内注射和皮内基因枪递送相比，通过电穿孔施用HPV DNA疫苗引起最强的CD8 + T细胞免疫反应。
3. Two Doses of Bovine Viral Diarrhea Virus DNA Vaccine Delivered by Electroporation Induce Long-Term Protective Immune Responses [J] . Sylvia van Drunen Littel-van den Hurk, Zoe Lawman, Marlene Snider, Clinical and vaccine immunology: CVI . 2013,第2期

机译：通过电穿孔递送两剂牛病毒性腹泻病毒DNA疫苗可引起长期保护性免疫反应。
4. INTRADERMAL ADMINISTRATION OF SYNTHETIC DNA VACCINES INDUCE ROBUST CELLULAR AND HUMORAL IMMUNE RESPONSES [C] . Jean D. Boyer Conference on vaccine technology VI . 2018

机译：皮内管理合成DNA疫苗诱导强健的细胞和体液免疫反应
5. Modulation of the immune response at systemic and mucosal sites by DNA vaccine adjuvants in HIV and influenza models. [D] . Kraynyak, Kimberly Ann. 2009

机译：DNA疫苗佐剂在HIV和流感模型中对全身和粘膜部位免疫应答的调节。
6. DNA immunization site determines the level of gene expression and the magnitude, but not the type of the induced immune response [O] . Stefan Petkov, Elizaveta Starodubova, Anastasia Latanova, 2012

机译：DNA免疫部位决定基因表达的水平和大小，但不能决定诱导的免疫反应的类型
7. The site of administration influences both the type and the magnitude of the immune response induced by DNA vaccine electroporation. [O] . Vandermeulen, Gaëlle, Vanvarenberg, Kevin, De Beuckelaer, Ans, 2015

机译：给药部位会影响DNA疫苗电穿孔诱导的免疫反应的类型和强度。
8. Multi-Agent Alphavirus DNA Vaccine Delivered by Intramuscular Electroporation Elicits Robust and Durable Virus Specific Immune Responses in Mice and Rabbits and Completely Protects Mice against Lethal Venezuelan, Western, and Eastern Equine Encephalitis Virus Aerosol Challenges. [R] . Dupuy, L. C., Richards, M. J., Livingston, B. D., 2016

机译：通过肌内电穿孔提供的多剂甲病毒DNa疫苗在小鼠和家兔中引发健壮和耐久的病毒特异性免疫应答，并完全保护小鼠免受致命的委内瑞拉，西方和东方马脑炎病毒气溶胶挑战。

The site of administration influences both the type and the magnitude of the immune response induced by DNA vaccine electroporation

摘要

著录项

相似文献

相关主题

期刊订阅