...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Vaccine >Detection and isolation of Bluetongue virus from commercial vaccine batches
【24h】

Detection and isolation of Bluetongue virus from commercial vaccine batches

机译：从商业疫苗批次中检测和分离蓝舌病毒

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this report we describe the detection and identification of Bluetongue virus (BTV) contaminations in commercial vaccines. BTV RNA was detected in vaccine batches of Lumpy skin disease (LSD) and Sheep pox (SP) using quantitative PCR (qPCR) for VP1 and NS3 genes. Both batches were positive for VP1 and NS3 in qPCR. The LSD vaccine-derived sample was positive for VP1 and VP2 in conventional PCR. The SP vaccine-derived sample was examined by amplification of VP1, VP4, VP6, VP7, NS2 and NS3 gene segments in conventional PCR. The SP vaccine-derived sample was further propagated in embryonated chicken eggs (ECE) and Vero cells. Preliminary sequence analysis showed that the LSD vaccine-derived sequence was 98-99% similar to BTV9. Analysis of the six genomic segments from the SP vaccine-derived isolate showed the highest similarity to BTV26 (66.3-97.8%). These findings are particularly important due to the effect of BTV on cattle and sheep, for which the vaccines are intended. They also demonstrate the necessity of rigorous vaccine inspection and strict vaccine production control. (C) 2016 Elsevier Ltd. All rights reserved.
机译：在本报告中，我们描述了商业疫苗中蓝舌病毒（BTV）污染的检测和鉴定。使用针对VP1和NS3基因的定量PCR（qPCR）在块状皮肤病（LSD）和绵羊痘（SP）疫苗批次中检测到BTV RNA。在qPCR中，这两个批次的VP1和NS3均为阳性。 LSD疫苗来源的样品在常规PCR中VP1和VP2呈阳性。在常规PCR中，通过扩增VP1，VP4，VP6，VP7，NS2和NS3基因片段来检查SP疫苗衍生的样品。 SP疫苗衍生的样品进一步在胚胎鸡蛋（ECE）和Vero细胞中繁殖。初步序列分析表明，LSD疫苗衍生的序列与BTV9相似，为98-99％。对来自SP疫苗的分离株的六个基因组片段的分析显示与BTV26的相似性最高（66.3-97.8％）。由于BTV对牛和羊具有疫苗的作用，因此这些发现特别重要。他们还证明了严格的疫苗检查和严格的疫苗生产控制的必要性。 （C）2016 Elsevier Ltd.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号