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首页> 外文期刊>Dalton transactions: An international journal of inorganic chemistry >Adsorption and detection of Escherichia coli using an Au substrate modified with a catecholate-type artificial siderophore-Fe~(3+) complex
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Adsorption and detection of Escherichia coli using an Au substrate modified with a catecholate-type artificial siderophore-Fe~(3+) complex

机译：邻苯二酚型人工铁-Fe〜（3+）配合物修饰的Au底物对大肠杆菌的吸附和检测

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摘要

A catecholate-type artificial siderophore with a terminal-NH_2 group (1) and its Fe~(3+) complex (2) were prepared. Siderophore 1 was characterized by 1H NMR, FT-IR, and ESI-TOF MS spectroscopy. The corresponding Fe~(3+) complex 2 was obtained by reaction of 1 with Fe(acac)_3. The absorption band at 500 nm (ε = 4670 M~(-1) cm~(-1) at pH 7.0) of the electronic absorption spectrum of 2 is assignable as the LMCT (O_(catecholate)→ Fe~(3+)) absorption band. This band indicates the formation of the Fe~(3+) complex of 1. The biological activity of 2 with respect to Escherichia coli was clearly confirmed by observing that it permeates into the cell membrane. The self-assembled monolayer of 2 on an Au substrate, 2/Au, was prepared and its preparation was confirmed by FT-IR reflection-absorption spectroscopy (IR-RAS) and cyclic voltammetry (CV). Furthermore, a quartz crystal microbalance (QCM) chip modified with 2 effectively adsorbed E. coli. M. flavescens, an organism which is incapable of synthesizing siderophores and must therefore use exogenous hydroxamate-type siderophores for growth, did not adsorb on 2/Au. In contrast, E. coli did not adsorb on the hydroxamate-type artificial siderophore-Fe 3+ complex (3)-modified Au substrate, 3/Au. These results provide preliminary evidence that microbes recognized Fe~(3+) ion-bound siderophores on the surface. The detection limit of 2/Au was ~10~4 CFU mL~(-1).
机译：制备了具有末端NH_2基团的儿茶酚型人工铁载体（1）及其Fe〜（3+）配合物（2）。铁载体1的特征在于1 H NMR，FT-IR和ESI-TOF MS光谱。通过使1与Fe（acac）_3反应获得相应的Fe〜（3+）配合物2。 LMCT（O_（儿茶酚）→Fe〜（3+）在500 nm处的吸收带（在pH 7.0下ε= 4670 M〜（-1）cm〜（-1）在pH 7.0时） ）吸收带。该条带表明形成了1的Fe〜（3+）配合物。通过观察其渗透到细胞膜中清楚地证实了2对大肠杆菌的生物活性。制备了在Au基板上2的自组装单分子层2 / Au，并通过FT-IR反射吸收光谱（IR-RAS）和循环伏安法（CV）确认了其制备。此外，用2种有效吸附的大肠杆菌修饰的石英微天平（QCM）芯片。苦参分支杆菌是一种无法合成铁载体的生物，因此必须使用外源异羟肟酸酯型铁载体进行生长，但它不会吸附在2 / Au上。相反，大肠杆菌没有吸附在异羟肟酸酯型人工铁载体-Fe 3+络合物（3）修饰的Au底物3 / Au上。这些结果为微生物识别表面的Fe〜（3+）离子结合铁载体提供了初步证据。 2 / Au的检出限为〜10〜4 CFU mL〜（-1）。

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