Metallo-regulation of the bimolecular triplex formation of a peptide nucleic acid

Shimada H.; Sakurai T.; Kitamura Y.; Matsuura H.; Ihara T.

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Metallo-regulation of the bimolecular triplex formation of a peptide nucleic acid

机译：肽核酸双分子三链体形成的金属调控

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Peptide nucleic acid (PNA) conjugates incorporating a bipyridine unit were prepared. The bipyridine was built into the loop moiety of PNAs that were designed to specifically form a hairpin and a PNA/DNA bimolecular triplex. While the thermal stability of the hairpin structure was only minimally affected by Cu~(2+) addition, the PNA/DNA bimolecular triplex structure was significantly destabilized by complexation with Cu~(2+). The melting temperature of the bimolecular triplex decreased by 17.4 °C in the presence of Cu~(2+). This corresponds to more than a 1000 fold decrease in the binding constant for bimolecular triplex formation. Upon complexation, the bipyridine unit underwent a drastic conformational change which accounts for the observed differences in the thermal stabilities of the triplex upon binding. The bipyridine-PNA conjugate may be useful as an allosteric DNA carrier that releases the DNA in response to a certain metal ion concentration.

机译：制备掺入联吡啶单元的肽核酸（PNA）缀合物。将联吡啶内置到PNA的环部分中，该环被设计成专门形成发夹和PNA / DNA双分子三链体。虽然发夹结构的热稳定性仅受Cu〜（2+）的影响最小，但PNA / DNA双分子三链体结构却因与Cu〜（2+）的复合作用而明显失稳。在Cu〜（2+）存在下，双分子三链体的熔化温度降低了17.4°C。这对应于双分子三链体形成的结合常数降低超过1000倍。络合后，联吡啶单元发生剧烈的构象变化，这解释了结合后三链体的热稳定性的观察到的差异。联吡啶-PNA缀合物可用作变构DNA载体，其响应于特定金属离子浓度而释放DNA。

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《Dalton transactions: An international journal of inorganic chemistry》 |2013年第45期|共8页
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Shimada H.; Sakurai T.; Kitamura Y.; Matsuura H.; Ihara T.;
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Binding constant; Bipyridine units; Bipyridines;

机译：结合常数;联吡啶单元;联吡啶;

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1. Metallo-regulation of the bimolecular triplex formation of a peptide nucleic acid [J] . Shimada H., Sakurai T., Kitamura Y., Dalton transactions: An international journal of inorganic chemistry . 2013,第45期

机译：肽核酸双分子三链体形成的金属调控
2. 2′-O,4′-C-Aminomethylene-Bridged Nucleic Acid Modification with Enhancement of Nuclease Resistance Promotes Pyrimidine Motif Triplex Nucleic Acid Formation at Physiological pH [J] . Prof. Hidetaka Torigoe, Dr. S. M. Abdur Rahman, Hiroko Takuma, Chemistry - A European Journal . 2011,第9期

机译：2'-O，4'-C-氨基亚甲基桥连核酸的核酸酶抗性修饰，在生理pH下促进嘧啶基序三链核酸的形成
3. 2'-O,4'-C-aminomethylene-bridged nucleic acid modification with enhancement of nuclease resistance promotes pyrimidine motif triplex nucleic acid formation at physiological pH. [J] . Torigoe H., Rahman S.M., Takuma H., Chemistry: A European journal . 2011,第9期

机译：具有增强的核酸酶抗性的2'-O，4'-C-氨基亚甲基桥接的核酸修饰在生理pH下促进嘧啶基序三链体核酸的形成。
4. Enzymatic ligation of peptides, peptide nucleic acids and proteins by means of sortase A [C] . Stephan Pritz, Yvonne Wolf, Oliver Kraetke American Peptide Symposium . 2009

机译：肽，肽核酸和蛋白质通过分子A的酶促结扎
5. Triplex-forming peptide nucleic acids as gene modifying agents at the beta-globin locus. [D] . Chin, Joanna Yee King. 2010

机译：形成三链体的肽核酸作为β-珠蛋白基因座的基因修饰剂。
6. Triplex formation with 2′-O4′-C-ethylene-bridged nucleic acids (ENA) having C3′-endo conformation at physiological pH [O] . Makoto Koizumi, Koji Morita, Makiko Daigo, 2003

机译：在生理pH下使用具有C3-内构象的2-O4-C-乙烯桥联核酸（ENA）形成三链体
7. Synthetic, Structural, and RNA Binding Studies on 2‐Aminopyridine‐Modified Triplex‐Forming Peptide Nucleic Acids [O] . Venubabu Kotikam, Scott D. Kennedy, James A. MacKay, 2019

机译：对2-氨基吡啶改性的三重复合肽核酸的合成，结构和RNA结合研究
8. Prebiotic Synthesis of Ethylenediamine Monoacetic Acid, The Repeating Unit of Peptide Nucleic Acids [R] . Nelson, K. E. , Miller, S. L. 1992

机译：益生元合成乙二胺单乙酸，肽核酸的重复单位

Metallo-regulation of the bimolecular triplex formation of a peptide nucleic acid

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