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首页> 外文期刊>Dalton transactions: An international journal of inorganic chemistry >Nuclease activity and interaction studies of unsymmetrical binuclear Ni(II) complexes with CT-DNA and BSA
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Nuclease activity and interaction studies of unsymmetrical binuclear Ni(II) complexes with CT-DNA and BSA

机译：不对称双核镍（II）与CT-DNA和BSA的核酸酶活性和相互作用研究

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摘要

New unsymmetrical binuclear nickel(II) complexes [Ni2L1-5] (ClO4)(2) (1-5) were synthesized by using [NiL] [(3-((9E)-(2-((E)-(3-formyl-2-olato-5-methylbenzylideneamino)methyl) phenylimino)methyl)-3-formyl-5-methyl-2-olato)nickel(II)] with various diamines like 1,2-diaminoethane (L-1), 1,3-diaminopropane (L-2), 1,4-diaminobutane (L-3), 1,2-diaminobenzene (L-4) and 1,8-diaminonaphthalene (L-5) and characterized by elemental analysis and spectroscopic methods. The molecular structure of binuclear nickel(II) complex 1 is determined by a single crystal X-ray diffraction method. Cyclic voltammograms of binuclear Ni(II) complexes exhibit two quasi-reversible reduction waves in the cathodic region and two oxidation waves in the anodic region. DNA binding, protein binding and DNA cleavage studies were investigated. The interactions of complexes (1-5) with calf thymus DNA were studied by spectroscopic techniques, including absorption and fluorescence methods. The binding affinities of complexes (1-5) with CT-DNA and nuclease activities are in the following order: 5 > 4 > 3 > 2 > 1. Binuclear Ni(II) complex 1 cleaved the plasmid DNA by a hydrolytic pathway. The hydrolytic cleavage of DNA by the complexes is supported by evidence from free radical quenching and T4 ligase ligation. Binuclear Ni(II) complexes (1-5) displayed significant protein (bovine serum albumin) interactions. The experimental results showed that the interaction between binuclear Ni(II) complexes and BSA was mainly a static quenching process.
机译：新的不对称双核镍（II）络合物[Ni2L1-5]（ClO4）（2）（1-5）通过使用[NiL] [[3-（（9E）-（2-（（E）-（3 -甲酰基-2-olato-5-甲基亚苄基氨基）甲基）苯基亚氨基）甲基）-3-甲酰基-5-甲基-2-olato）镍（II）]与各种二胺，如1,2-二氨基乙烷（L-1）， 1,3-二氨基丙烷（L-2），1,4-二氨基丁烷（L-3），1,2-二氨基苯（L-4）和1,8-二氨基萘（L-5）并通过元素分析和光谱法表征方法。双核镍（II）配合物1的分子结构通过单晶X射线衍射法确定。双核Ni（II）配合物的循环伏安图在阴极区域显示两个准可逆还原波，在阳极区域显示两个氧化波。研究了DNA结合，蛋白质结合和DNA切割研究。配合物（1-5）与小牛胸腺DNA的相互作用通过光谱技术进行了研究，包括吸收法和荧光法。配合物（1-5）与CT-DNA的结合亲和力和核酸酶活性的顺序如下：5> 4> 3> 2> 1. Binuclear Ni（II）复合物1通过水解途径切割质粒DNA。自由基猝灭和T4连接酶连接的证据支持复合物对DNA的水解切割。双核Ni（II）配合物（1-5）显示出显着的蛋白质（牛血清白蛋白）相互作用。实验结果表明，双核Ni（II）配合物与BSA之间的相互作用主要是静态猝灭过程。

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