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首页> 外文期刊>Dalton transactions: An international journal of inorganic chemistry >New bimetallic palladium(II) and platinum(II) complexes: studies of the nucleophilic substitution reactions, interactions with CT-DNA, bovine serum albumin and cytotoxic activity
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New bimetallic palladium(II) and platinum(II) complexes: studies of the nucleophilic substitution reactions, interactions with CT-DNA, bovine serum albumin and cytotoxic activity

机译：新的双金属钯（II）和铂（II）配合物：亲核取代反应，与CT-DNA的相互作用，牛血清白蛋白和细胞毒性活性的研究

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Two new dinuclear bimetallic complexes, [{PdCl(bipy)}{mu-(pyrazine)}{PtCl(bipy)}]Cl(ClO4) (1) (bipy is 2,2'-=bipyridine) and [{PdCl(en)}{mu-(pyrazine)}{PtCl(en)}]Cl(ClO4) (2) (en is ethylenediamine), have been synthesized and characterized by elemental microanalysis, IR, H-1 NMR spectroscopy and MALDI-TOF mass spectrometry. The pK(a) values of the coordinated water molecules of the diaqua species were determined as well. Substitution reactions of complexes (1) and (2) with thiourea (Tu), L-methionine (L-Met), L-cysteine (L-Cys), L-histidine (L-His) and guanosine-5'-monophosphate (5'-GMP) were studied under the pseudo-first order conditions as a function of nucleophile concentration and temperature. The order of reactivity of nucleophiles was: Tu > L-Met > L-Cys > L-His > 5'-GMP. Substitution reactions with Tu, L-Cys and L-His were followed by decomposition of bimetallic complexes to the corresponding substituted mononuclear complexes [Pd(N-N)(Nu)(2)] and [Pt(N-N)(Nu)(2)] (N-N = bipy, en), releasing the bridging ligand. However, the structures of starting bimetallic complexes were preserved during the reactions with L-Met and 5'-GMP. The absorption spectroscopic study of interactions of calf-thymus DNA (CT-DNA) with complexes (1), (2) and [{PdCl(bipy)}{mu-(NH2(CH2)(6)H2N)} {PtCl(bipy)}] Cl(ClO4) (3), has shown that all the complexes exhibit high intrinsic binding constants (K-b = 10(4)-10(5) M-1). DNA-ethidium bromide (DNA-EB) fluorescence was quenched after addition of complexes (1), (2) or (3), indicating displacement of intercalating EB by complexes. All complexes have shown good binding affinity to bovine serum albumin protein (BSA). Chemosensitivity of A375 (human melanoma) and HeLa (human cervical cancer) cell lines toward complexes (1), (2) and (3) was analyzed by SRB assay. Complex (1) displayed significant inhibitory effect on the growth of both cell lines.
机译：两个新的双核双金属配合物[{PdCl（bipy）} {mu-（吡嗪）} {PtCl（bipy）}] Cl（ClO4）（1）（bipy是2,2'-= bipyridine）和[{PdCl（ en）} {mu-（吡嗪）} {PtCl（en）}] Cl（ClO4）（2）（en是乙二胺），已经通过元素微分析，IR，H-1 NMR光谱和MALDI-TOF合成并表征质谱。还确定了恶臭物种的配位水分子的pK（a）值。配合物（1）和（2）与硫脲（Tu），L-蛋氨酸（L-Met），L-半胱氨酸（L-Cys），L-组氨酸（L-His）和鸟苷5'-单磷酸的取代反应（5'-GMP）在拟一级反应条件下作为亲核试剂浓度和温度的函数进行研究。亲核试剂的反应顺序为：Tu> L-Met> L-Cys> L-His> 5'-GMP。用Tu，L-Cys和L-His取代反应后，双金属配合物分解为相应的取代单核配合物[Pd（NN）（Nu）（2）]和[Pt（NN）（Nu）（2）] （NN = bipy，en），释放桥联配体。但是，在与L-Met和5'-GMP反应期间，起始的双金属配合物的结构得以保留。小牛胸腺DNA（CT-DNA）与复合物（1），（2）和[{PdCl（bipy）} {mu-（NH2（CH2）（6）H2N）} {PtCl（ Bipy）}] Cl（ClO4）（3）表明，所有配合物均显示出高固有结合常数（Kb = 10（4）-10（5）M-1）。添加复合物（1），（2）或（3）后，DNA-溴化乙锭（DNA-EB）荧光被淬灭，表明复合物取代了嵌入的EB。所有复合物均显示出与牛血清白蛋白（BSA）的良好结合亲和力。通过SRB测定法分析了A375（人黑素瘤）和HeLa（人宫颈癌）细胞系对复合物（1），（2）和（3）的化学敏感性。复合物（1）对两种细胞系的生长均显示出显着的抑制作用。

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