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首页> 外文期刊>Current Biology: CB >Specialization of a Drosophila Capping Protein Essential for the Protection of Sperm Telomeres
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Specialization of a Drosophila Capping Protein Essential for the Protection of Sperm Telomeres

机译：果蝇加帽蛋白的保护精子端粒必不可少的专业化。

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Background: A critical function of telomeres is to prevent fusion of chromosome ends by the DNA repair machinery. In Drosophila somatic cells, assembly of the protecting capping complex at telomeres notably involves the recruitment of HOAP, HP1, and their recently identified partner, Hip Hop. We previously showed that the hiphop gene was duplicated before the radiation of the melanogaster subgroup of species, giving birth to K81, a unique paternal effect gene specifically expressed in the male germline.Results: Here we show that K81 specifically associates with telomeres during spermiogenesis, along with HOAP and HP1, and is retained on paternal chromosomes until zygote formation. In K81 mutant testes, capping proteins are not maintained at telomeres in differentiating spermatids, resulting in the transmission of uncapped paternal chromosomes that fail to properly divide during the first zygotic mitosis. Despite the apparent similar capping roles of K81 and Hip Hop in their respective domain of expression, we demonstrate by in vivo reciprocal complementation analyses that they are not interchangeable. Strikingly, Hip Hop appeared to be unable to maintain capping proteins at telomeres during the global chromatin remodeling of spermatid nuclei.Conclusions: Our data demonstrate that K81 is essential for the maintenance of capping proteins at telomeres in postmeiotic male germ cells. In species of the melanogaster subgroup, Hip Hop and K81 have not only acquired complementary expression domains, they have also functionally diverged following the gene duplication event. We propose that K81 specialized in the maintenance of telomere protection in the highly peculiar chromatin environment of differentiating male gametes.
机译：背景：端粒的关键功能是防止DNA修复机制融合染色体末端。在果蝇的体细胞中，保护性封端复合物在端粒的组装主要涉及HOAP，HP1及其最近鉴定的伴侣Hip Hop的募集。先前我们已经证明，hiphop基因在辐射黑猩猩亚组之前被复制，从而产生了K81，这是一种在雄性种系中特异性表达的独特的父本效应基因。结果：在这里，我们表明K81在精子发生过程中与端粒特异性结合，以及HOAP和HP1，并保留在父本染色体上，直到合子形成。在K81突变型睾丸中，未在分化的精子细胞的端粒上保留封端蛋白，从而导致未封端的父本染色体的传播，该染色体在第一次合子有丝分裂期间无法正确分裂。尽管K81和Hip Hop在它们各自的表达域中具有明显相似的加帽作用，但我们通过体内相互互补分析证明它们是不可互换的。令人惊讶的是，在精子细胞核的整体染色质重塑期间，Hip Hop似乎无法维持端粒的加帽蛋白。结论：我们的数据表明K81对于维持减数分裂后雄性生殖细胞端粒的加帽蛋白至关重要。在黑腹果蝇亚组的物种中，Hip Hop和K81不仅获得了互补的表达域，而且在基因复制事件后它们的功能也有所不同。我们建议K81专门在分化雄性配子的高度特殊染色质环境中维持端粒保护。

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