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首页> 外文期刊>Cryo Letters >CHELATION OF TRACE ELEMENTS IN PRESERVATION MEDIUM INFLUENCES THE QUALITY OF BOAR SPERMATOZOA DURING LIQUID PRESERVATION AT 5 degrees C FOR 4 WEEKS
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CHELATION OF TRACE ELEMENTS IN PRESERVATION MEDIUM INFLUENCES THE QUALITY OF BOAR SPERMATOZOA DURING LIQUID PRESERVATION AT 5 degrees C FOR 4 WEEKS

机译：保存介质中微量元素的变化影响在5摄氏度下保存4周的液体中公猪精子的质量

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BACKGROUND: The addition of a metal chelator, ethylenediaminetetraacetic acid (EDTA), to semen extender has the purpose of capturing trace element ions. OBJECTIVE: This study was conducted to evaluate the effects of EDTA on the quality and in vitro fertilisability of liquid-preserved boar spermatozoa. METHODS: In Experiment 1, semen samples were preserved in the semen extender supplemented with 0, 3, 6, or 12 mM of Na-EDTA at 5 degrees C for 4 weeks. In Experiment 2, semen samples were preserved in the extender supplemented with 3 mM of Na-EDTA, Ca-EDTA, or Zn-EDTA and without chelator EDTA. RESULTS: When Na-EDTA was used as a chelating substance in the extender, 3 mM was a most suitable concentration for sperm motility and viability after cold preservation. The supplementation of 3 mM Ca-EDTA had advantages regarding sperm motility, viability and plasma membrane integrity. CONCLUSION: Our findings indicate that 3 mM Ca-EDTA is the most suitable metal-chelating substance for the liquid preservation of boar semen.
机译：背景：在精液补充剂中添加金属螯合剂乙二胺四乙酸（EDTA）的目的是捕获痕量元素离子。目的：本研究旨在评价EDTA对液体保存的公猪精子质量和体外受精能力的影响。方法：在实验1中，将精液样品在补充有0、3、6或12 mM Na-EDTA的精液补充剂中于5摄氏度保存4周。在实验2中，将精液样品保存在补充了3 mM Na-EDTA，Ca-EDTA或Zn-EDTA且没有螯合剂EDTA的补充剂中。结果：当使用Na-EDTA作为增量剂中的螯合剂时，3 mM是冷藏后精子活力和活力的最合适浓度。补充3 mM Ca-EDTA在精子活力，生存力和质膜完整性方面具有优势。结论：我们的发现表明3 mM Ca-EDTA是最适合用于猪精液液体保存的金属螯合物质。

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