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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Antioxidative activity of volatile extracts isolated from Angelica tenuissimae roots, peppermint leaves, pine needles, and sweet flag leaves
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Antioxidative activity of volatile extracts isolated from Angelica tenuissimae roots, peppermint leaves, pine needles, and sweet flag leaves

机译：当归根，薄荷叶，松针和甜旗叶中的挥发性提取物的抗氧化活性

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摘要

Volatile extracts were isolated from dried medicinal plants [Angelica tenuissimae roots (AT, Angelica tenuissima Nakai), peppermint leaves (PL, Mentha arvensis L.), pine needles (PN, Pinus sylvestris L.), and sweet flag leaves (SF, Acorus gramineus Rhizoma)] using steam distillation under reduced pressure, followed by continuous liquid-liquid extraction (DRP-LLE). The extracts were then analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The major volatile constituents of AT, PL, PN, and SF were 3-butylidene-4,5-dihydrophthalide (32 mg/g), menthol (18 mg/g), thunbergol (2.1 mg/g), and cis-asarone (37 mg/g), respectively. The inhibitory activity (%) of the extracts against hexanal oxidation ranged from 33 to 98% at a level of 50 μ g/mL. Among the volatile extracts, PL and PN increased cell viabilities by 10 and 24%, respectively, at a dose of 1 μ g/mL, compared to that of H2O2-treated brain neuroblastoma cells, SK-N-SH. However, a 20% reduction in the malonaldehyde level, an index for lipid peroxidation, was observed at only 1 μ g/mL concentration of PN.
机译：从干燥的药用植物[当归根（AT，当归当归中），薄荷叶（PL，薄荷），松针（PN，樟子松）和甜旗叶（SF，Acorus）中分离挥发物草根茎]]，在减压下使用水蒸气蒸馏，然后连续进行液-液萃取（DRP-LLE）。然后通过气相色谱（GC）和气相色谱-质谱（GC-MS）分析提取物。 AT，PL，PN和SF的主要挥发性成分为3-丁叉-4,5-二氢邻苯二甲酸酯（32 mg / g），薄荷醇（18 mg / g），秋柏酚（2.1 mg / g）和顺式去（37 mg / g）。提取物对己醛氧化的抑制活性（％）在50μg / mL的水平范围内为33％至98％。在挥发性提取物中，与H2O2处理的脑神经母细胞瘤细胞SK-N-SH相比，PL和PN以1μg / mL的剂量分别将细胞活力提高了10％和24％。但是，仅在1μg / mL的PN浓度下，丙二醛水平（脂质过氧化的指标）降低了20％。

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