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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Monitoring of monooctanoylphosphatidylcholine synthesis by enzymatic acidolysis between soybean phosphatidylcholine and caprylic acid by thin-layer chromatography with a flame ionization detector
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Monitoring of monooctanoylphosphatidylcholine synthesis by enzymatic acidolysis between soybean phosphatidylcholine and caprylic acid by thin-layer chromatography with a flame ionization detector

机译：火焰离子化检测器薄层色谱法检测大豆磷脂酰胆碱和辛酸之间的酶促酸解，监测单辛酰基磷脂酰胆碱的合成

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Thin-layer chromatography with a flame ionization detector (TLC-FID) was used for monitoring the production of structured phospholipids (ML type: L, long-chain fatty acids; M, medium-chain fatty acids) by enzyme-catalyzed acidolysis between soybean phosphatidylcholine (PC) and caprylic acid. It was found that the structured PC fractionated into two to three distinct bands on both plate thin-layer chromatography (TLC) and Chromarod TLC. These three bands represented PC of the LL type, ML type, and MM type, respectively. The TLC-FID method was applied in the present study to examine the influence of enzyme dosage, reaction temperature, solvent amount, reaction time, and substrate ratio (caprylic acid/PC, mol/mol) on formation of ML-type PC in a batch reactor with Thermomyces lanuginosa lipase as the catalyst. The formation of ML-type PC was dependent on all parameters examined except for the substrate ratio. The ML-type PC content increased with increasing enzyme dosage, reaction temperature, solvent amount, and reaction time. The substrate ratio had no significant effect on the formation of ML-type PC within the tested range (3-15 mol/mol). The formation of MM-type PC was observed in some experiments, indicating that acyl migration is taking place during reaction since the lipase is claimed to be 1,3-specific. The TLC-FID method offers a simple and cheap technique for elucidation of product and byproduct formation during enzyme-catalyzed reactions for production of phospholipids containing mixtures of long- and medium-chain fatty acids.
机译：使用火焰离子化检测器（TLC-FID）进行的薄层色谱法通过大豆之间的酶催化酸解监测结构化磷脂（ML类型：L，长链脂肪酸; M，中链脂肪酸）的产生磷脂酰胆碱（PC）和辛酸。发现在板薄层色谱（TLC）和Chromarod TLC上结构化的PC分为两到三个不同的谱带。这三个频带分别代表LL型，ML型和MM型的PC。本研究采用TLC-FID方法检测酶用量，反应温度，溶剂量，反应时间和底物比例（辛酸/ PC，摩尔/摩尔）对ML型PC形成的影响。以嗜热霉菌脂肪酶为催化剂的间歇反应器。 ML型PC的形成取决于所检查的所有参数，除了底物比例。 ML型PC含量随酶用量，反应温度，溶剂量和反应时间的增加而增加。在测试范围（3-15mol / mol）内，底物比例对ML型PC的形成没有显着影响。在某些实验中观察到MM型PC的形成，这表明由于脂肪酶被认为是1,3特异性的，因此在反应过程中发生了酰基迁移。 TLC-FID方法提供了一种简单而廉价的技术，可用于阐明酶催化反应过程中产物和副产物的形成，以生产包含长链和中链脂肪酸混合物的磷脂。

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