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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Effect of ginsenosides on glucose uptake in human Caco-2 cells is mediated through altered Na+/glucose cotransporter 1 expression.
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Effect of ginsenosides on glucose uptake in human Caco-2 cells is mediated through altered Na+/glucose cotransporter 1 expression.

机译：人参皂甙对人Caco-2细胞摄取葡萄糖的影响是通过改变Na + /葡萄糖共转运蛋白1的表达来介导的。

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摘要

In this study, we measured the effect of ginsenosides on glucose uptake using the Caco-2 cell system. At submicromolar concentrations, these compounds exhibited marked effects on the rate of glucose transport across the differentiated Caco-2 cell monolayer. Compound K (CK), the main intestinal bacterial metabolite of the protopanaxadiol ginsenosides, significantly enhanced the steady-state glucose transport rate to about 50% of the control sample rate (from 1.54 +/- 0.09 to 2.25 +/- 0.15 nmol/min). Conversely, the protopanaxatriol ginsenoside Rg1 inhibited glucose transport to about 70% of the original rate (from 1.54 +/- 0.09 to 1.02 +/- 0.05 nmol/min). Consistent with the effect on glucose uptake rate, CK and Rg1 conferred a significant and paralleled alteration on both the protein and mRNA expression levels of the Na+/glucose cotransporter 1 (SGLT1) gene. Unlike SGLT1, there is no significant alteration on the protein or mRNA levels of GLUTs in CK- or Rg1-treated cells. Taken together, our results demonstrate that ginsenosides CK and Rg1 elicited potent enhancing and suppressing effects, respectively, on glucose uptake across human intestinal Caco-2 monolayer through modulation of SGLT1 expression.
机译：在这项研究中，我们使用Caco-2细胞系统测量了人参皂甙对葡萄糖摄取的影响。在亚微摩尔浓度下，这些化合物对跨分化的Caco-2细胞单层的葡萄糖转运速率显示出显着影响。化合物K（CK）是原托那沙糖人参皂苷的主要肠道细菌代谢产物，可将稳态葡萄糖转运速率显着提高至对照样品速率的约50％（从1.54 +/- 0.09到2.25 +/- 0.15 nmol / min ）。相反，前托那沙三醇人参皂苷Rg1抑制葡萄糖转运至原始速率的约70％（从1.54 +/- 0.09到1.02 +/- 0.05 nmol / min）。与对葡萄糖摄取率的影响一致，CK和Rg1赋予了Na + /葡萄糖共转运蛋白1（SGLT1）基因的蛋白质和mRNA表达水平显着且平行的改变。与SGLT1不同，在CK或Rg1处理的细胞中，GLUT的蛋白质或mRNA水平没有明显改变。两者合计，我们的结果表明，人参皂苷CK和Rg1分别通过调节SGLT1表达，对整个人肠Caco-2单层葡萄糖摄取产生了有效的增强和抑制作用。

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