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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Induction of apoptosis by the Lactuca indica L. in human leukemia cell line and its active components.
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Induction of apoptosis by the Lactuca indica L. in human leukemia cell line and its active components.

机译：Lactuca indica L.在人白血病细胞系及其活性成分中诱导凋亡。

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Lactuca indica L. (Compositae family) is used as a folk medicine in anti-inflammatory, antibacterial, antidiabetic, and other medications in Asia. The objectives of this study were to evaluate the antiproliferative effect of ethanol extracts of Lactuca indica L. (EEL) on human leukemic HL-60 cell lines and its active components. The results showed that EEL exhibited strong cytotoxic effects against HL-60 cells; the IC50 value was 313 microg/mL. Flow cytometric analysis of the externalization of phosphatidylserine (PS) using the annexin V/PI method on EEL-treated HL-60 cells showed a concentration-dependent increase of apoptosis. Moreover, EEL could induce typical DNA fragmentation in a concentration- and time-dependent manner as determined by electrophoresis and TUNEL assays. The treatment of HL-60 cells with EEL induced significant accumulation of cells in the G0/G1 phase, indicating that EEL is a cell-cycle-dependent anticancer agent. Our results also indicate that EEL-induced apoptosis in HL-60 cells is associated with the loss of mitochondrial membrane potential (delta psi m). EEL contains 5% phenolic compounds, such as quercetin, caffeic acid, rutin, and chlorogenic acid. Among the four active phenolic compounds, quercetin was found to be the most effective in inhibition against cell viability and in alteration of mitochondrial function. Our results suggest that the induction of apoptosis by EEL might offer a pivotal mechanism for its chemopreventive action.
机译：Lactuca indica L.（菊科）在亚洲用作抗炎，抗菌，抗糖尿病和其他药物的民间药物。这项研究的目的是评估印度乳杆菌（Lactuca indica L.）（EEL）乙醇提取物对人白血病HL-60细胞系及其活性成分的抗增殖作用。结果表明，EEL对HL-60细胞具有较强的细胞毒性作用。 IC50值为313微克/毫升。使用膜联蛋白V / PI方法对EEL处理的HL-60细胞进行的磷脂酰丝氨酸（PS）外在化的流式细胞仪分析显示，凋亡的浓度依赖性增加。此外，EEL可以通过浓度和时间依赖性的方式诱导典型的DNA片段化，这是通过电泳和TUNEL分析确定的。用EEL处理HL-60细胞可导致G0 / G1期细胞大量积聚，表明EEL是一种依赖细胞周期的抗癌剂。我们的结果还表明，EEL诱导的HL-60细胞凋亡与线粒体膜电位（δpsi m）的损失有关。 EEL包含5％的酚类化合物，例如槲皮素，咖啡酸，芦丁和绿原酸。在四种活性酚类化合物中，槲皮素在抑制细胞活力和改变线粒体功能方面最有效。我们的结果表明，EEL诱导细胞凋亡可能为其化学预防作用提供关键机制。

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