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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Optimization of Acid Hydrolysis Conditions for Feruloylated Oligosaccharides from Rice Bran through Response Surface Methodolgy
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Optimization of Acid Hydrolysis Conditions for Feruloylated Oligosaccharides from Rice Bran through Response Surface Methodolgy

机译：响应面法优化米糠中阿魏酸寡糖的酸水解条件

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摘要

Response surface methodology(RSM)was employed to optimize the hydrolysis conditions with trifluoroacetic acid(TFA)to obtain the maximum amount of feruloylated oligosaccharides from rice bran.The TFA concentration and hydrolysis time effects on feruloylated oligosaccharides recovery are studied.The optimum hydrolysis conditions for maximizing feruloylated oligosaccharides recovery were 193 mM TFA concentration and 1.36 h of hydrolysis time.Under these conditions the corresponding acyl ferulic group quantity was 78.63 mu g in 1 mL of hydrolysate.The model was experimentally verified with a satisfactory coefficient of R~2(=0.96).The quantity of acyl ferulic group in the feruloylated oligosaccharides,purified using Amberlite XAD-4,was 916.12 mu g/g of rice bran under the optimum hydrolysis conditions.The proposed method accounted for 54.08% of the total acyl ferulic group in rice bran.The results suggest that the proposed conditions were useful in maximizing recovery of feruloylated oligosaccharides from rice bran.
机译：采用响应面法（RSM）优化了三氟乙酸（TFA）的水解条件，获得了米糠中阿魏酸酯化寡糖的最大量，研究了TFA浓度和水解时间对阿魏酸酯化寡糖回收率的影响。阿魏酸寡糖的最大回收率为193 mM TFA浓度和1.36 h的水解时间。在这些条件下，相应的酰基阿魏酸基团量为1 mL水解物中78.63μg。实验验证了该模型，具有令人满意的R〜2（= 0.96）。在最佳水解条件下，使用Amberlite XAD-4纯化的阿魏酸酯化寡糖中的酰基阿魏基团含量为916.12μg/ g米糠，拟议的方法占米糠中总酰基阿魏基团的54.08％结果表明，所提出的条件有助于最大限度地提高阿魏酸酯化寡核苷酸的回收率米糠中的糖精。

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