Toward fish and seafood traceability: Anchovy species determination in fish products by molecular markers and support through a public domain database

Jerome M; Martinsohn JT; Ortega D; Carreau P; Verrez-Bagnis V; Mouchel O

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Toward fish and seafood traceability: Anchovy species determination in fish products by molecular markers and support through a public domain database

机译：促进鱼类和海鲜的可追溯性：通过分子标记确定鱼产品中的An鱼种类，并通过公共领域的数据库提供支持

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Traceability in the fish food sector plays an increasingly important role for consumer protection and confidence building. This is reflected by the introduction of legislation and rules covering traceability on national and international levels. Although traceability through labeling is well established and supported by respective regulations, monitoring and enforcement of these rules are still hampered by the lack of efficient diagnostic tools. We describe protocols using a direct sequencing method based on 212-274-bp diagnostic sequences derived from species-specific mitochondria DNA cytochrome b, 16S rRNA, and cytochrome oxidase subunit I sequences which can efficiently be applied to unambiguously determine even closely related fish species in processed food products labeled "anchovy". Traceability of anchovy-labeled products is supported by the public online database AnchovyID (http://anchovyid.jrc.ec.europa.eu), which provided data obtained during our study and tools for analytical purposes.

机译：鱼类食品部门的可追溯性在保护消费者和建立信任方面发挥着越来越重要的作用。引入涵盖国家和国际层面可追溯性的立法和规则反映了这一点。尽管通过标签的可追溯性已得到相应法规的充分确立和支持，但是由于缺乏有效的诊断工具，这些规则的监视和执行仍然受到阻碍。我们描述了一种基于直接测序方法的协议，该方法基于源自物种特异性线粒体DNA细胞色素b，16S rRNA和细胞色素氧化酶亚基I序列的212-274-bp诊断序列，该序列可有效地用于明确确定甚至密切相关的鱼类加工食品标记为“ an鱼”。公开在线数据库AnchovyID（http://anchovyid.jrc.ec.europa.eu）支持cho鱼标记产品的可追溯性，该数据库提供了我们在研究过程中获得的数据以及用于分析目的的工具。

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《Journal of Agricultural and Food Chemistry》 |2008年第10期|共10页
作者
Jerome M; Martinsohn JT; Ortega D; Carreau P; Verrez-Bagnis V; Mouchel O;
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正文语种 eng
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species identification; food product; anchovy; Clupeomorpha; Engraulis; cytochrome b gene; cytochrome oxidase subunit I gene; 16S rRNA gene; direct sequencing; FINS; public domain database; POLYMERASE-CHAIN-REACTION; MITOCHONDRIAL CYTOCHROME-B; DIRECT SEQUENCING METHOD; ENGRAULIS-ENCRASICOLUS; CONTROL REGION; DNA SEQUENCE; CANNED TUNA; PCR-RFLP; IDENTIFICATION; ATLANTIC;

机译：种类鉴定;食品;an鱼;Cl形;En草;细胞色素b基因;细胞色素氧化酶亚基I基因;16S rRNA基因;直接测序;FINS;公共领域数据库;聚合物链反应;线粒体细胞色素B;直接测序法;恩氏菌;对照区;DNA序列;罐头金枪鱼;PCR-RFLP;鉴定;大西洋;

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专利

1. Toward fish and seafood traceability: Anchovy species determination in fish products by molecular markers and support through a public domain database [J] . Jerome M, Martinsohn JT, Ortega D, Journal of Agricultural and Food Chemistry . 2008,第10期

机译：促进鱼类和海鲜的可追溯性：通过分子标记确定鱼产品中的An鱼种类，并通过公共领域的数据库提供支持
2. The importance of distinguishing pufferfish species (Lagocephalus spp.) in the Mediterranean Sea for ensuring public health: Evaluation of the genetic databases reliability in supporting species identification [J] . Giusti Alice, Guarducci Marcella, Stern Nir, Fisheries Research . 2019,第期

机译：区分河豚物种（Lagocephalus SPP）在地中海的重要性，确保公共卫生：评估遗传数据库在支持物种鉴定方面的可靠性
3. Contributions (in 2005) of marine and fresh water products (finfish and shellfish, seafood, wild and farmed) to the French dietary intakes of vitamins D and B12, selenium, iodine and docosahexaenoic acid: impact on public health. [J] . Edouard Bourre JM, Marc Paquotte P International Journal of Food Sciences and Nutrition . 2008,第6期

机译：海洋和淡水产品（鱼类和贝类，海鲜，野生和养殖产品）（2005年）对法国饮食中维生素D和B12，硒，碘和二十二碳六烯酸的摄入的贡献：对公共健康的影响。
4. DIRECT SEQUENCING METHOD FOR MOLECULAR IDENTIFICATION OF CANNED ANCHOVY AND ANCHOVY-TYPE PRODUCTS: SHORT 16S MITOCHONDRIAL DNA FRAGMENT MARKER [C] . I. Guarniero, P.P. Gatta, S. Testi, International Conference of the European Aquaculture Society . 2004

机译：罐头锚杆和锚型产品分子鉴定的直接测序方法：短16S线粒体DNA片段标记
5. A historical perspective of California recreational fisheries using a new database of "trophy" fish records (1966-2013), combined with fisheries analyses of three species in the genus Paralabrax. [D] . Bellquist, Lyall F. 2015

机译：使用“奖杯”鱼类记录的新数据库（1966-2013年），结合对Paralabrax属中三种鱼类的渔业分析，对加州休闲渔业进行了历史考察。
6. Erucic Acid (22:1n-9) in Fish Feed Farmed and Wild Fish and Seafood Products [O] . Nini H. Sissener, Robin Ørnsrud, Monica Sanden, 2018

机译：鱼饲料养殖的野生鱼和海鲜产品中的芥酸（22：1n-9）
7. Proposal for a Regulation (EEC) of the Council fixing the guide prices for the fishery products listed in Annex I (A) and (C) to Regulation (EEC) No 2142/70 for the 1976 fishing year. Proposal for a Regulation (EEC) of the Council fixing the guide prices for fishery products listed in Annex II to Regulation (EEC) No 2142/70 for the 1976 fishing year. Proposal for a Regulation (EEC) of the Council fixing intervention prices for fresh or chilled sardines and anchovies for the 1976 fishing year. Proposal for a Regulation (EEC) of the Council fixing the Community producer price for tunny intended for the canning industry for the 1976 fishing year. Proposal for a Regulation (EEC) of the Council supplementing Regulation (EEC) No 2455/70 determining common marketing standards for certain fresh or chilled fish. COM (75) 614 final, 24 November 1975 [O] . 1975

机译：关于1976年捕捞年度法规（EEC）No 2142/70附件I（a）和（C）所列渔业产品指导价格的理事会条例（EEC）的提案。关于1976年捕捞年度法规（EEC）No 2142/70附件II所列渔业产品指导价格的理事会条例（EEC）的提案。关于理事会法规（EEC）的提案，确定1976年捕捞年份新鲜或冷藏沙丁鱼和凤尾鱼的干预价格。关于理事会法规（EEC）的提案，该法规确定1976年捕捞年度用于罐头行业的金枪鱼的共同体生产者价格。关于理事会补充条例（EEC）No 2455/70的法规（EEC）的提案，确定某些新鲜或冷冻鱼的共同营销标准。 COm（75）614决赛，1975年11月24日
8. Species Profiles: Life Histories and Environmental Requirements of Coastal Fishes and Invertebrates (Gulf of Mexico) - Bay Anchovy and Striped Anchovy [R] . Robinette, H. R. 1983

机译：物种概况：沿海鱼类和无脊椎动物（墨西哥湾）的生活历史和环境要求 - 海湾凤尾鱼和条纹凤尾鱼

Toward fish and seafood traceability: Anchovy species determination in fish products by molecular markers and support through a public domain database

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